CAJ | 학술논문

采用循环伏安法、微分脉冲伏安法、计时库仑法并结合紫外光谱法研究了一种电中性多吡啶铜配合物[Cu（phen）（PC）（H2O）]（phen=1,10-邻菲罗啉,PC=2,6-吡啶二羧酸）与DNA的相互作用.紫外吸收光谱显示,配合物溶液加入DNA后,配合物的特征吸收峰减小,产生明显的减色效应,表明二者发生了相互作用.循环伏安结果表明配合物在玻碳电极呈现一对准可逆氧化还原峰.加入DNA后,配合物的峰电流明显减小,式电位发生正移,表明二者可能通过嵌插方式发生作用.微分脉冲伏安法考察了不同浓度的DNA对配合物的影响,表明以[Cu（phen）（PC）（H2O）]作为电化学探针,能在3.3×10–6 mol/L~4.0×10–5 mol/L浓度范围内对DNA进行定量检测.计时库仑法进一步显示,配合物与DNA形成复合物后扩散系数明显降低了.
채용순배복안법、미분맥충복안법、계시고륜법병결합자외광보법연구료일충전중성다필정동배합물[Cu（phen）（PC）（H2O）]（phen=1,10-린비라람,PC=2,6-필정이최산）여DNA적상호작용.자외흡수광보현시,배합물용액가입DNA후,배합물적특정흡수봉감소,산생명현적감색효응,표명이자발생료상호작용.순배복안결과표명배합물재파탄전겁정현일대준가역양화환원봉.가입DNA후,배합물적봉전류명현감소,식전위발생정이,표명이자가능통과감삽방식발생작용.미분맥충복안법고찰료불동농도적DNA대배합물적영향,표명이[Cu（phen）（PC）（H2O）]작위전화학탐침,능재3.3×10–6 mol/L~4.0×10–5 mol/L농도범위내대DNA진행정량검측.계시고륜법진일보현시,배합물여DNA형성복합물후확산계수명현강저료.
The interaction between a polypyridyl copper complex Cu（phen）（PC）（H2O）（phen=1,10-phenan-throline,PC=2,6-pyridyl-dicarboxylato） and DNA was investigated by electrochemical methods and UV spectrophotometry.With addition of DNA,the characteristic absorption peak of Cu（phen）（PC）（H2O） was weakened,and a hypochromism was occurred,demonstrating the interaction between the copper complex and DNA.It was showed that the complex was an electroactive compound at glassy carbon electrode with a pair of quasi-reversible redox peaks.The interaction of DNA with Cu（phen）（PC）（H2O） caused the significant decrease of redox peak currents coupled with positive shift of formal potentials,indicating that Cu（phen）（PC）（H2O） might bind with DNA by an intercalation mode.Differential pulse voltammetry showed that the concentration of DNA could be qualified in the range of 3.3×10-6 mol/L ~ 4.0×10-5 mol/L via using Cu（phen）（PC）（H2O） as an electroactive probe.Chronocoulometry suggested that the diffusion rate of the complex was decreased obviously after interaction with DNA.