CAJ | 학술논문

利用ISSR分子标记技术对40份玉米自交系进行亲缘关系分析。从59条ISSR引物中筛选出10条重复性高、多态性好的引物,分别对全部供试材料进行扩增,共扩出90条清晰谱带,其中多态性条带81条,多态性比率为90.00%,表明供试材料基因组DNA的多态性较高。用NTSYSpc-2.10软件中的UPGMA进行聚类分析,40份玉米自交系的遗传相似系数变化范围在0.65～1.00之间,在此基础上构建聚类分析树状图,揭示了玉米各自交系间的亲缘关系。本研究为今后分子水平上玉米优良自交系的改良与选育提供了一定的参考依据。
이용ISSR분자표기기술대40빈옥미자교계진행친연관계분석。종59조ISSR인물중사선출10조중복성고、다태성호적인물,분별대전부공시재료진행확증,공확출90조청석보대,기중다태성조대81조,다태성비솔위90.00%,표명공시재료기인조DNA적다태성교고。용NTSYSpc-2.10연건중적UPGMA진행취류분석,40빈옥미자교계적유전상사계수변화범위재0.65～1.00지간,재차기출상구건취류분석수상도,게시료옥미각자교계간적친연관계。본연구위금후분자수평상옥미우량자교계적개량여선육제공료일정적삼고의거。
Genetic relationship of 40 maize inbred lines were analyzed by inter-simple sequence repeat （ISSR） marker.Ten primers,which selected from 59 primers,were used to amplify the maize simple,90 bands were amplified,including 81 polymorphic bands.The percentage of polymorphic bands was 90.00%.The results showed that plenty diversities existed in the maize inbred lines.Cluster analysis by UPGMA indicated that genetic similarity was from 0.65～1.00.The result revealed the genetic relationship between different maise inbred lines.All the result provide reference for to fine varieties improvement and breeding on molecular level.