CAJ | 학술논문

采集海南大学苦丁茶种质资源库中6种冬青科(Tlex)苦丁茶树的根、茎、叶及对应土壤样品,通过试验研究,建立了高压密封消解-石墨炉原子吸收光谱法测定样品中镉的方法.对植物样和土壤样分别采用硝酸-双氧水和硝酸-高氯酸-氢氟酸混酸体系进行消解.以磷酸二氢铵作为基体改进剂,并开启氘灯校正背景,在优化的工作条件下测定样品中镉.该方法的检出限为0.300ng/mL,用于实验样品中镉的测定,其相对标准偏差RSD＜5%,加标回收率介于94%～108%之间.用该方法对茶叶(GBW10016)和土壤(GBW-07407)标准参考物中镉进行了测定,测定结果与标准值相符合,相对误差小于7%.冬青科苦丁茶树及其土壤中镉的分布具有特征性,主要表现为:土壤＞＞茶树,根际土壤＞非根际土壤,吸收根＞主根或茎,嫩叶＞老叶＞枯叶.
채집해남대학고정다충질자원고중6충동청과(Tlex)고정다수적근、경、협급대응토양양품,통과시험연구,건립료고압밀봉소해-석묵로원자흡수광보법측정양품중력적방법.대식물양화토양양분별채용초산-쌍양수화초산-고록산-경불산혼산체계진행소해.이린산이경안작위기체개진제,병개계도등교정배경,재우화적공작조건하측정양품중력.해방법적검출한위0.300ng/mL,용우실험양품중력적측정,기상대표준편차RSD＜5%,가표회수솔개우94%～108%지간.용해방법대다협(GBW10016)화토양(GBW-07407)표준삼고물중력진행료측정,측정결과여표준치상부합,상대오차소우7%.동청과고정다수급기토양중력적분포구유특정성,주요표현위:토양＞＞다수,근제토양＞비근제토양,흡수근＞주근혹경,눈협＞로협＞고협.
Root, stem, leaf and corresponding soil samples of 6 kinds of Kudingcha trees in germplasm resource bank of Hainan University were studied by experiment, and high pressure sealed digestion-graphite furnace atomic absorption spectrometry (GF-AAS) was used to determine Cd in samples, with detection limit of 0.300ng'mL-1, RSD less than 5%,recovery test rate between 89%-108%. The method was used to determine standard reference materials of tea and soil, with results in conformity with the standard of GBW10016 and GBW-07407, and relative error less than 5%. Distribution of cadmium in Kudingcha trees and soil was in the order as soil, Kudingcha trees, rhizosphere soil, non-rhizosphere soil,absorbing roots, main root or stem, young leaves, old leaves and dead leaves.