CAJ | 학술논문

从具有同步硝化反硝化(SND)现象的OGO反应器中分离出三株好氧反硝化菌(分别命名为T3、T6、T7),经测定其革兰氏染色皆为阳性且均为杆状.通过分析形态学特征和16S rD-NA同源性比较,确定T3、T7为赤红红球菌属,T6为戈登氏菌属.菌株在以柠檬酸钠为碳源、硝酸钾为氮源的培养基中生长良好,将其按比例混合扩大培养后以5%的接种量投放于OGO反应器中,检测了菌株强化后反应器的脱氮效果.结果表明:聚乙烯醇(PVA)包埋菌泥投放较菌泥直接投放的处理效果好,经其强化后OGO反应器对COD、NH_4~+-N、TN的平均去除率分别为98.48%、90.18%、78.92%,比强化前分别提高了4.21%、6.43%、4.61%,且反应器出水中的NO_x~--N含量较低.
종구유동보초화반초화(SND)현상적OGO반응기중분리출삼주호양반초화균(분별명명위T3、T6、T7),경측정기혁란씨염색개위양성차균위간상.통과분석형태학특정화16S rD-NA동원성비교,학정T3、T7위적홍홍구균속,T6위과등씨균속.균주재이저몽산납위탄원、초산갑위담원적배양기중생장량호,장기안비례혼합확대배양후이5%적접충량투방우OGO반응기중,검측료균주강화후반응기적탈담효과.결과표명:취을희순(PVA)포매균니투방교균니직접투방적처리효과호,경기강화후OGO반응기대COD、NH_4~+-N、TN적평균거제솔분별위98.48%、90.18%、78.92%,비강화전분별제고료4.21%、6.43%、4.61%,차반응기출수중적NO_x~--N함량교저.
Three strains of aerobic denitrifying bacteria were isolated from the OGO reactor with simultaneous nitrification and denitrification (SND) phenomenon, and named as T3, T6 and T7. They are all Gram-positive and rod-like. T3 and T7 are identified as Rhodococcus and T6 as Gordonia according to their morphological characteristics and 16S rDNA sequence homology comparison. The strains grow well in the culture medium with potassium nitrate as nitrogen source and sodium citrate as carbon source. After enlarging cultivation, the bacteria were added into the OGO reactor with inoculated amount of 5%. The nitrogen removal efficiency was tested in the OGO reactor enhanced with the aerobic denitrifying bacteria. The results indicate that the bacteria immobilized by polyvinyl alcohol (PVA) are superior to free (non-immobilized) bacteria in treatment efficiency. The average removal rates of COD, ammonia nitrogen and total nitrogen in the enhanced reactor are 98.48% , 90. 18% and 78.92% respectively, being 4.21% , 6.43% and 4.61% higher than those in the non-enhanced reactor. Moreover, NO_x~- - N in the effluent is lower.