CAJ | 학술논문

目的通过观察针刀松解法对兔膝骨关节炎模型行为学和形态学的影响,探讨骨性关节炎的发病机理及针刀松解法治疗的可行性.方法以膝关节伸直位固定法造成膝骨关节炎模型,用电针和针刀进行治疗,治疗3周后观察Lequesne MG的膝关节评估级别,取胫骨组织HE染色后进行光镜观察,切取股骨内髁软骨进行透射电镜观察.结果 Lequesne MG评分显示在局部反应、步态反应和关节活动上,电针组和针刀组分别与正常组、模型组比有显著性差异(P＜0.05),电针组和针刀组之间比较无显著性差异(P＞0.05).HE染色显示电针组和针刀组软骨组织损伤减轻,且有修复趋势;电镜结果显示针刀组兔膝关节软骨细胞破坏程度和胶原纤维紊乱程度明显减轻,与电针组相当.结论针刀松解法对膝骨关节炎有较好的治疗效果,从宏观到微观证实了针刀松解法的有效性.
목적 통과관찰침도송해법대토슬골관절염모형행위학화형태학적영향,탐토골성관절염적발병궤리급침도송해법치료적가행성.방법 이슬관절신직위고정법조성슬골관절염모형,용전침화침도진행치료,치료3주후관찰Lequesne MG적슬관절평고급별,취경골조직HE염색후진행광경관찰,절취고골내과연골진행투사전경관찰.결과 Lequesne MG평분현시재국부반응、보태반응화관절활동상,전침조화침도조분별여정상조、모형조비유현저성차이(P＜0.05),전침조화침도조지간비교무현저성차이(P＞0.05).HE염색현시전침조화침도조연골조직손상감경,차유수복추세;전경결과현시침도조토슬관절연골세포파배정도화효원섬유문란정도명현감경,여전침조상당.결론 침도송해법대슬골관절염유교호적치료효과,종굉관도미관증실료침도송해법적유효성.
Objective To investigate the pathogenesis of osteoarthritis and feasibility of acupotomy therapy through the observation on the influences of acupotomy therapy on ethology and morphology in rabbits with knee osteoarthritis.Methods The model of knee osteoarthritis was established by applying the fixed method of knee extension posture in rabbits. The therapy of electro-acupuncture and acupotomy therapy were used to treat the rabbits. After 3 weeks the knee joint function was observed and reviewed based on Lequesne algofunctional index. The light microscope was used to observe tibia tissue after HE staining. The transmission electron microscope (TEM) was used to observe the internal femoral condyle cartilage.Results Lequesne algofunctional index showed that in local reaction, gait reaction and joint motion there was a significant different between the electro-acupuncture group or acupotomy group and the normal group or model group (P<0.05), respectively, while there was no different between the electro-acupuncture group and acupotomy group (P>0.05). HE staining showed that the cartilage injury was relieved and tended to recovery in the electro-acupuncture group and acupotomy group. The result of TEM showed that the lesion of cartilage cells and disorder of collagen fibrils were relieved in the acupotomy group, which is similar to those in the electro-acupuncture group.Conclusion The acupotomy therapy has a good curative effect on knee osteoarthritis, which is proved at macroscopic and microscopic levels.