中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2009年
21期
2735-2738
,共4页
齐晓勇%孟存良%李英肖%党懿%刘惠良%王天红%袁华兵%倪艳辉%刘光%武振朝%郜利会
齊曉勇%孟存良%李英肖%黨懿%劉惠良%王天紅%袁華兵%倪豔輝%劉光%武振朝%郜利會
제효용%맹존량%리영초%당의%류혜량%왕천홍%원화병%예염휘%류광%무진조%고리회
腹主动脉%动脉粥样硬化%炎性反应%白介素-8%单克隆抗体
腹主動脈%動脈粥樣硬化%炎性反應%白介素-8%單剋隆抗體
복주동맥%동맥죽양경화%염성반응%백개소-8%단극륭항체
Abdominal aorta%Atherosclerosis%Inflammatory reaction%Interleukin-8%Monoclonal antibody
目的 观察白介素-8(IL-8)水平对兔腹主动脉粥样硬化狭窄的影响.方法 新西兰大白兔36只随机分为实验组、IL-8单克隆抗体治疗组、对照组.实验组和治疗组动物通过穿刺股动脉送入球囊拉伤腹主动脉.治疗组动物通过耳缘静脉注射IL-8单克隆抗体进行干预.对照组动物只进行股动脉穿刺和留置鞘管,不进行球囊扩张拉伤.分别于实验前,实验后4 h、1 d、3 d,1 w、2 w、4 w,耳缘静脉采血4 ml,检测三组动物血清IL-8水平,4 w后处死动物进行病理组织学检查,应用光学显微镜和计算机病理图像分析系统分析测定以下指标:内膜中膜厚度、面积,计算血管狭窄程度.结果 实验组动物球囊损伤4 h后IL-8水平即开始升高,术后1 d达到峰值,持续增高4 w,且明显高于治疗组和对照组.治疗组和对照组动物IL-8水平无上述变化规律.实验组动物腹主动脉明显狭窄,组织学检查内膜面积和中膜面积、血管狭窄程度等指标大于治疗组和对照组(P<0.01),治疗组管腔狭窄,对照组动物管腔无明显变化,治疗组与对照组比较无显著性差异(P>0.05).相关分析显示,IL-8水平与内膜厚度、内膜面积、中膜厚度、中膜面积等呈正相关(相关系数r分别为0.894,0.783,0.801,0.912,均P<0.01).结论 IL-8水平高表达引起腹主动脉粥样硬化狭窄的产生,经IL-8单克隆抗体治疗后动脉狭窄程度明显减轻.IL-8水平与动脉狭窄程度呈正相关,是发生腹主动脉粥样硬化狭窄的独立危险因素.
目的 觀察白介素-8(IL-8)水平對兔腹主動脈粥樣硬化狹窄的影響.方法 新西蘭大白兔36隻隨機分為實驗組、IL-8單剋隆抗體治療組、對照組.實驗組和治療組動物通過穿刺股動脈送入毬囊拉傷腹主動脈.治療組動物通過耳緣靜脈註射IL-8單剋隆抗體進行榦預.對照組動物隻進行股動脈穿刺和留置鞘管,不進行毬囊擴張拉傷.分彆于實驗前,實驗後4 h、1 d、3 d,1 w、2 w、4 w,耳緣靜脈採血4 ml,檢測三組動物血清IL-8水平,4 w後處死動物進行病理組織學檢查,應用光學顯微鏡和計算機病理圖像分析繫統分析測定以下指標:內膜中膜厚度、麵積,計算血管狹窄程度.結果 實驗組動物毬囊損傷4 h後IL-8水平即開始升高,術後1 d達到峰值,持續增高4 w,且明顯高于治療組和對照組.治療組和對照組動物IL-8水平無上述變化規律.實驗組動物腹主動脈明顯狹窄,組織學檢查內膜麵積和中膜麵積、血管狹窄程度等指標大于治療組和對照組(P<0.01),治療組管腔狹窄,對照組動物管腔無明顯變化,治療組與對照組比較無顯著性差異(P>0.05).相關分析顯示,IL-8水平與內膜厚度、內膜麵積、中膜厚度、中膜麵積等呈正相關(相關繫數r分彆為0.894,0.783,0.801,0.912,均P<0.01).結論 IL-8水平高錶達引起腹主動脈粥樣硬化狹窄的產生,經IL-8單剋隆抗體治療後動脈狹窄程度明顯減輕.IL-8水平與動脈狹窄程度呈正相關,是髮生腹主動脈粥樣硬化狹窄的獨立危險因素.
목적 관찰백개소-8(IL-8)수평대토복주동맥죽양경화협착적영향.방법 신서란대백토36지수궤분위실험조、IL-8단극륭항체치료조、대조조.실험조화치료조동물통과천자고동맥송입구낭랍상복주동맥.치료조동물통과이연정맥주사IL-8단극륭항체진행간예.대조조동물지진행고동맥천자화류치초관,불진행구낭확장랍상.분별우실험전,실험후4 h、1 d、3 d,1 w、2 w、4 w,이연정맥채혈4 ml,검측삼조동물혈청IL-8수평,4 w후처사동물진행병리조직학검사,응용광학현미경화계산궤병리도상분석계통분석측정이하지표:내막중막후도、면적,계산혈관협착정도.결과 실험조동물구낭손상4 h후IL-8수평즉개시승고,술후1 d체도봉치,지속증고4 w,차명현고우치료조화대조조.치료조화대조조동물IL-8수평무상술변화규률.실험조동물복주동맥명현협착,조직학검사내막면적화중막면적、혈관협착정도등지표대우치료조화대조조(P<0.01),치료조관강협착,대조조동물관강무명현변화,치료조여대조조비교무현저성차이(P>0.05).상관분석현시,IL-8수평여내막후도、내막면적、중막후도、중막면적등정정상관(상관계수r분별위0.894,0.783,0.801,0.912,균P<0.01).결론 IL-8수평고표체인기복주동맥죽양경화협착적산생,경IL-8단극륭항체치료후동맥협착정도명현감경.IL-8수평여동맥협착정도정정상관,시발생복주동맥죽양경화협착적독립위험인소.
Objective To observe the effects of interleukin-8 (IL-8) on atherosclerosis (AS) of abdominal aorta in rabbits. Methods Thirty-six New Zealand white rabbits were randomly divided into experiment, treatment and control groups, each group 12 cases. Rabbits in experiment and treatment groups were injuried in abdominal aorta by balloon inflation after punctured in femoral artery. Monoclonal antibody of IL-8 was injected by venous in rabbits of treatment group. Rabbits in control group were punctured only in femoral artery. Peripheral blood was collected before experiment and 4h, 1,3d, 1, 2, 4 w later. The level of IL-8 was measured by enzyme linked immunosorbent assay ( ELISA) respectively. The luminals of abdomial aorta in three groups were observed 4 w later. Histopalhologic examination was performed. Such contents as follows were assayed by light microscope and computer image analysis system; luminal area, intima and tunica media area, angiostenosis of blood vessel. Results The level of II.-8 was raised in 4 h and achieved to peak in 1 d after balloon inflation in rabbits of experiment group. The higher level of this inflammatory factor lasted 4 w. There was no variation above in treatment and control groups. It was observed that abdominal aorta became stenosis obviously in experiment group. The area of intima and tunica media as well as the extent of stenosis in experiment group were bigger than those in treatment and control groups. There was no statistical difference between treatment and control groups. Correlation analysis indicated that there were positive relations between IL-8 and thickness, area of intima and tunica medias respectively(r =0. 894, 0.783,0. 801,0. 912,P <0. 01). Multiple regression analysis of Logistic indicated that IL-8 was an independent risk for the AS(P<0.01). The high levels of IL-8 leaded to AS in abdominal aorta of rabbits. The severity of stenosis became lessen after intervention by monoclonal antibody of IL-8. Positive corelation was found between the level of IL-8 and severity of arterial stenosis. IL-8 was one of independent risks factor leading occurrence and development of AS. Conclusions The high levels of IL-8 leads to AS in abdominal aorta of rabbits. The severity of stenosis becomes lessen after intervention by monoclonal antibody of IL-8. Positive corelation is found between the level of IL-8 and severity of arterial stenosis. IL-8 is one of independent risks factor leading occurrence and development of AS.
