CAJ | 학술논문

目前应用显微镜技术很难进行鱼卵种类的准确鉴定,本文尝试采用DNA技术开展鱼卵的鉴定工作.以带鱼(Trichiurus haumela) 鱼卵为研究对象,采用苯酚-氯仿法提取了其DNA基因组,并采用PCR及克隆测序的方法,对其18S rDNA序列进行了测定与初步分析.结果表明,提取的带鱼(T.haumela) 鱼卵DNA基因组片段长度约23 kb,PCR扩增片段长度约1.8 kb;带鱼(T.haumela) 鱼卵18S rDNA与其它已知鱼类的18S rDNA序列具有一定的差异性,可以用其进行带鱼(T.haumela) 鱼卵的初步鉴定.
목전응용현미경기술흔난진행어란충류적준학감정,본문상시채용DNA기술개전어란적감정공작.이대어(Trichiurus haumela) 어란위연구대상,채용분분-록방법제취료기DNA기인조,병채용PCR급극륭측서적방법,대기18S rDNA서렬진행료측정여초보분석.결과표명,제취적대어(T.haumela) 어란DNA기인조편단장도약23 kb,PCR확증편단장도약1.8 kb;대어(T.haumela) 어란18S rDNA여기타이지어류적18S rDNA서렬구유일정적차이성,가이용기진행대어(T.haumela) 어란적초보감정.
For the moment,it is difficult to distinguish different species of fish eggs with microscopical technique,and that DNA technique was applied to identify species of fish eggs in the paper.The Trichiurus haumela fish eggs was tested and its genome was extracted with the method of phenol-chloroform and its 18S rDNA sequence was also studied with PCR and clone technique.The results showed that the gene sequence length of the Trichiurus haumela fish eggs was about 23 kb,and that the sequence length of PCR product was about 1.8 kb.The 18S rDNA sequence of the T.haumela fish egg was different from other known fishes,showing that 18S rDNA could be used to identify T.haumela fish egg.