福建农林大学学报(自然科学版)
福建農林大學學報(自然科學版)
복건농림대학학보(자연과학판)
JOURNAL OF FUJIAN AGRICULTURE AND FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
2009年
5期
465-470
,共6页
蔡学清%鄢凤娇%林玉%胡方平
蔡學清%鄢鳳嬌%林玉%鬍方平
채학청%언봉교%림옥%호방평
西瓜细菌性果斑病%拮抗%内生细菌%芽孢杆菌
西瓜細菌性果斑病%拮抗%內生細菌%芽孢桿菌
서과세균성과반병%길항%내생세균%아포간균
Acidovorax avenae subsp. citrulli%antagonism%endophytic bacteria%Bacillus spp.
从葫芦科作物及空心菜的叶片、茎及花上共分离到95个菌株,采用抑菌圈法共筛选到对西瓜细菌性果斑病病原菌具有不同程度拮抗作用的菌株46株,占分离菌株总数的48.4%;从拮抗菌株中筛选出5株对西瓜细菌性果斑病菌具有较好拮抗效果的菌株,其对西瓜细菌性果斑病的防治效果为68.75%-93.24%.内生性测定结果表明这5株菌株均能在西瓜体内定殖,5株菌株经过革兰氏染色、鞭毛染色、芽孢染色和一些生理生化测定,初步被确定为芽孢杆菌属(Bacillus spp.).
從葫蘆科作物及空心菜的葉片、莖及花上共分離到95箇菌株,採用抑菌圈法共篩選到對西瓜細菌性果斑病病原菌具有不同程度拮抗作用的菌株46株,佔分離菌株總數的48.4%;從拮抗菌株中篩選齣5株對西瓜細菌性果斑病菌具有較好拮抗效果的菌株,其對西瓜細菌性果斑病的防治效果為68.75%-93.24%.內生性測定結果錶明這5株菌株均能在西瓜體內定殖,5株菌株經過革蘭氏染色、鞭毛染色、芽孢染色和一些生理生化測定,初步被確定為芽孢桿菌屬(Bacillus spp.).
종호호과작물급공심채적협편、경급화상공분리도95개균주,채용억균권법공사선도대서과세균성과반병병원균구유불동정도길항작용적균주46주,점분리균주총수적48.4%;종길항균주중사선출5주대서과세균성과반병균구유교호길항효과적균주,기대서과세균성과반병적방치효과위68.75%-93.24%.내생성측정결과표명저5주균주균능재서과체내정식,5주균주경과혁란씨염색、편모염색、아포염색화일사생리생화측정,초보피학정위아포간균속(Bacillus spp.).
Ninety five bacterial strains were isolated from water spinach (Ipomaea aquatica) and Cucurbitaceae plants, of which forty six endophytic bacterial strains could inhibit Acidovorax avenae subsp. Citrulli, it accounted for 48.4%. Five strains were selected with the control effect of 68.75% -93.24%.The five strains could colonize in watermelon, and were identified as Bacillus spp. Based on the results of Cram, flagella, spore stains and physiological and biochemical tests.