材料导报
材料導報
재료도보
MATERIALS REVIEW
2009年
14期
106-108
,共3页
邹泽昌%韦奇%孙慧%纳薇%聂祚仁
鄒澤昌%韋奇%孫慧%納薇%聶祚仁
추택창%위기%손혜%납미%섭조인
介孔泡沫材料%木瓜蛋白酶%酶固定化%热稳定性%储藏稳定性
介孔泡沫材料%木瓜蛋白酶%酶固定化%熱穩定性%儲藏穩定性
개공포말재료%목과단백매%매고정화%열은정성%저장은정성
siliceous mesocellulax foam%papain%enzyme immobilization%thermal stability%storage stability
以氧化硅介孔泡沫材料(Siliceous mesocellular foam,MCF)为载体对木瓜蛋白酶进行了固定,并对固定化后材料的表征、固定化酶的性能以及酶的稳定性进行了研究.结果表明,木瓜蛋白酶在MCF上能获得较大的固定量(334mg/g MCF)和较高的酶活,固定化酶活力为游离酶的95.3%.固定化酶和游离酶的米氏常数Km值分别为6.99×10-3mol/L和6.71×10-3mol/L,并且相对于游离酶固定化酶的热稳定性和储藏稳定性都有明显改善.MCF是一种良好的木瓜蛋白酶固定载体.
以氧化硅介孔泡沫材料(Siliceous mesocellular foam,MCF)為載體對木瓜蛋白酶進行瞭固定,併對固定化後材料的錶徵、固定化酶的性能以及酶的穩定性進行瞭研究.結果錶明,木瓜蛋白酶在MCF上能穫得較大的固定量(334mg/g MCF)和較高的酶活,固定化酶活力為遊離酶的95.3%.固定化酶和遊離酶的米氏常數Km值分彆為6.99×10-3mol/L和6.71×10-3mol/L,併且相對于遊離酶固定化酶的熱穩定性和儲藏穩定性都有明顯改善.MCF是一種良好的木瓜蛋白酶固定載體.
이양화규개공포말재료(Siliceous mesocellular foam,MCF)위재체대목과단백매진행료고정,병대고정화후재료적표정、고정화매적성능이급매적은정성진행료연구.결과표명,목과단백매재MCF상능획득교대적고정량(334mg/g MCF)화교고적매활,고정화매활력위유리매적95.3%.고정화매화유리매적미씨상수Km치분별위6.99×10-3mol/L화6.71×10-3mol/L,병차상대우유리매고정화매적열은정성화저장은정성도유명현개선.MCF시일충량호적목과단백매고정재체.
Siliceous mesocellulax foam(MCF) is employed as carriers in the immobilization of papain, and the properties and the stabilities of the immobilized enzyme axe investigated in detail. The results show that the amount of papain immobilized on MCF reaches 334mg/g MCF and the activity of immobilized papain is 95. 3% of that of the free papain. The Km of immobilized and free papain are 6. 99×10-3 mol/L and 6. 71×10-3mol/L, respectively. The thermal and storage stabilities of immobilized enzyme are improved due to the mieroenvironment arid the shield of the mesopores. MCF can be considered as excellent supports for papain immobilization.
