CAJ | 학술논문

目的探讨金雀异黄素(Gen)对高糖培养下大鼠系膜细胞(MC)表型和细胞外基质(ECM)的影响.方法对照组:低糖DMEM培养液;实验组:高糖DMEM培养液(含0、5、10、50、100、200 μmol/L Gen),培养12、24、48 h,分别检测SMemb mRNA、MMP-2 mRNA、TIMP-1 mRNA水平及上清液Fn的含量.结果 Gen可抑制高糖刺激下SMemb的表达,减少Fn的分泌(P＜0.01或0.05);并可增加MMP-2/TIMP-1mRNA半定量比值,呈浓度、时间依赖性.结论在体外高糖条件下,Gen干预可降低MC SMemb表达,减轻ECM积聚.
목적 탐토금작이황소(Gen)대고당배양하대서계막세포(MC)표형화세포외기질(ECM)적영향.방법 대조조:저당DMEM배양액;실험조:고당DMEM배양액(함0、5、10、50、100、200 μmol/L Gen),배양12、24、48 h,분별검측SMemb mRNA、MMP-2 mRNA、TIMP-1 mRNA수평급상청액Fn적함량.결과 Gen가억제고당자격하SMemb적표체,감소Fn적분비(P＜0.01혹0.05);병가증가MMP-2/TIMP-1mRNA반정량비치,정농도、시간의뢰성.결론 재체외고당조건하,Gen간예가강저MC SMemb표체,감경ECM적취.
Objective To evaluate the effect of genistein on the secretion of SMemb and on extracellular matrix of rat mesangial cells cultured in high glucose.Methods Control group(low glucose of 100mg/dl without genistein)and experiment group(high glucose of 450mg/dl with 0,5,10,50,100,200μmol/L of genistein)were incubated for 12,24,48 hrs.The expressions of SMemb mRNA,MMP-2 mRNA and TIMP-1 mRNA and synthesis of Fn were examined.Results Genistein inhibited the expression of SMemb mRNA,decreased Fn secretion(P<0.01 or 0.05).Genistein also increased the semi-quantitative ratio of MMP-2/TIMP-1 mRNA in dose-dependent and time-dependent manner.Conclusions Under hyperglycemia,genistein can decrease the overexpression of SMemb mRNA,and relieve accumulation of extracellular matrix.