中国化学工程学报（英文版）
中國化學工程學報（英文版）
중국화학공정학보（영문판）
CHINESE JOURNAL OF CHEMICAL ENGINEERING
2006年
1期
132-136
,共5页
秦加阳%肖梓军%马翠卿%谢能中%刘培海%许平
秦加暘%肖梓軍%馬翠卿%謝能中%劉培海%許平
진가양%초재군%마취경%사능중%류배해%허평
2,3-butanediol production,optimization,fed-batch,Klebsiella pneumoniae
The production of 2,3-butanediol by Klebsiella pneumoniae from glucose supplemented with different salts was studied. A suitable medium composition was defined by response surface experiments. In a medium containing glucose and (NH4)2HPO4, the strain could convert 137.0g of glucose into 52.4g of 2,3-butanediol and 8.4g of acetoin in shaking flasks. The diol yield amounted to 90％ of its theoretical value and the productivity was 1-1.5g·L-1·h-1. In fed-batch fermentation, the yield and productivity of diol were further enhanced by maintaining the pH at 6.0. Up to 92.4g of 2,3-butanediol and 13.1g of acetoin per liter were obtained from 215.0g of glucose per liter. The diol yield reached 98% of its theoretical value and the productivity was up to 2.1g·L-1·h-1.
