CAJ | 학술논문

在乙谜麻醉下,分别于明时(8:00 a.m.)及暗时(8:00 p.m.)断头处死野生型及组胺H1R基因敲除型小鼠,迅速取出脑组织并分离出皮层、纹状体、海马、下丘脑、丘脑、中脑及脑干等脑区.这些脑组织被制成匀浆并用HPLC荧光检测法测量其组胺含量.结果显示暗时处死时,H1R基因敲除型小鼠海马、丘脑、中脑及脑干中的组胺含量明显低于野生型小鼠.明时处死时,野生型小鼠各脑区组胺含量均较暗时处死显著降低,但这一变化在H1R基因敲除型小鼠中并未观察到.这些表明作为组胺的功能靶,H1R不仅介导组胺的功能,而且调节大脑中组胺含量与释放的昼夜节律.
재을미마취하,분별우명시(8:00 a.m.)급암시(8:00 p.m.)단두처사야생형급조알H1R기인고제형소서,신속취출뇌조직병분리출피층、문상체、해마、하구뇌、구뇌、중뇌급뇌간등뇌구.저사뇌조직피제성균장병용HPLC형광검측법측량기조알함량.결과현시암시처사시,H1R기인고제형소서해마、구뇌、중뇌급뇌간중적조알함량명현저우야생형소서.명시처사시,야생형소서각뇌구조알함량균교암시처사현저강저,단저일변화재H1R기인고제형소서중병미관찰도.저사표명작위조알적공능파,H1R불부개도조알적공능,이차조절대뇌중조알함량여석방적주야절률.
Under anesthesia by diethyl ether, histamine H1 receptor (H1R) gene knockout (KO) and wild-type (WT) mice were killed by decapitation either at 8:00 p.m. or at 8:00 a.m. The brains were quickly removed out and divided on ice into seven regions: the cortex, striatum, hippocampus, hypothalamus, thalamus, midbrain and medulla. These brain regions were homogenized and the histamine content in the homogenate was determined by HPLC- fluorometry. The results showed that at 8:00 p.m. histamine contents in the hippocampus, thalamus, midbrain, and medulla of DPR-KO mice were significantly decreased compared with WT mice (P<0.05). In WT mice, histamine contents in almost all brain regions were markedly higher at 8:00 p.m. than at 8:00 a.m, whereas the difference was not observed in H1R KO mice. We conclude that as the function target of histamine, H1R not only modulates the function of histamine, but also regulates the histamine content and its synthesizing or releasing rhythm in the mouse brain.