CAJ | 학술논문

背景:目前获取诱发电位的方法都是采用平均叠加的方法,这种方法不能动态连续观察病变的每一瞬态变化.新的自适应时间延迟估计方法(time delay estimation,TDE)克服了平均叠加方法的不足.目的:利用新的自适应时间延迟估计方法来观察缺氧过程中体感诱发电位(somatosensory evoked potentials,SEP)的瞬态潜伏期变化.设计:随机对照研究.地点和对象:实验地点:美国约翰霍普金斯大学、美国伊利诺大学生物医学信号分析实验室及大连理工大学电子与信息工程学院.实验动物为4只健康成年杂种猫,来源于美国约翰霍普金斯大学医学院动物室.干预:猫被麻醉后,SEP记录电极置于皮质感觉区硬膜外,在缺氧前、缺氧(80 mL/L O2)过程中及给氧(1 000 mL/L O2)后连续记录SEP,利用TDE方法分析SEP.主要观察指标:缺氧与SEP的瞬态潜伏期变化的关系.结果:以时间为横轴,以潜伏期变化为纵轴,可以很明了地观察到:在缺氧前SEP的潜伏期变化在零附近;80 mL/L O2条件下,潜伏期变化逐渐增大;1000 mL/L O2供给条件下,SEP潜伏期变化又逐渐趋向零附近.结论:这种新的TDE方法可以动态检测SEP的瞬态潜伏期变化,从而可以动态观察中枢神经系统病变的动态变化.
배경:목전획취유발전위적방법도시채용평균첩가적방법,저충방법불능동태련속관찰병변적매일순태변화.신적자괄응시간연지고계방법(time delay estimation,TDE)극복료평균첩가방법적불족.목적:이용신적자괄응시간연지고계방법래관찰결양과정중체감유발전위(somatosensory evoked potentials,SEP)적순태잠복기변화.설계:수궤대조연구.지점화대상:실험지점:미국약한곽보금사대학、미국이리낙대학생물의학신호분석실험실급대련리공대학전자여신식공정학원.실험동물위4지건강성년잡충묘,래원우미국약한곽보금사대학의학원동물실.간예:묘피마취후,SEP기록전겁치우피질감각구경막외,재결양전、결양(80 mL/L O2)과정중급급양(1 000 mL/L O2)후련속기록SEP,이용TDE방법분석SEP.주요관찰지표:결양여SEP적순태잠복기변화적관계.결과:이시간위횡축,이잠복기변화위종축,가이흔명료지관찰도:재결양전SEP적잠복기변화재령부근;80 mL/L O2조건하,잠복기변화축점증대;1000 mL/L O2공급조건하,SEP잠복기변화우축점추향령부근.결론:저충신적TDE방법가이동태검측SEP적순태잠복기변화,종이가이동태관찰중추신경계통병변적동태변화.
BACKGROUND: Average superposition technique is applied to acquire signals of evoked potentials(EP) in clinical practice, but it can't detect EP latency changes of every sweep in diseases. A new method of adaptive time delay estimation(TDE) can make up this shortcoming.OBJECTIVE: To observe the momentary latency changes of somatosensory evoked potentials(SEP) in hypoxia with the new TDE method.DESIGN: A randomized and controlled trial.SETTINGS and PARTICIPANTS: Experimental units: John Hopkins University of USA; Laboratory of Biomedical Signals Analysis, University of Illinois of USA; College of Electron and Information Engineering, Dalian University of Technology. Four healthy adult hybrid cats were selected from the Animal Laboratory, the Medical College of John Hopkins University of USA.INTERVENTIONS: After the anesthesia of cats, the recording electrode of SEP was placed outside the dura mater of cortex sensory area. SEP was recorded continuously before hypoxia, during hypoxia (80 mL/L O2) and after oxygen was provided( 1 000 mL/L O2) in order to analyze with TDE.MAIN OUTCOME MEASURES: The relationship between hypoxia and momentary latency changes of SEP.RESULTS: With time as the abscissa and the latency changes as the ardinate, it showed that the latency changes of SEP was close to zero before hypoxia, increased gradually under 80 mL/L O2 given, and was close to zero again under 1 000 mL/L O2 given.CONCLUSION: This new TDE method can detect instantaneous latency changes of SEP, so it can be used to observe diseases of central nervous system dynamically.