CAJ | 학술논문

目的建立流动注射抑制化学发光测定乙酰螺旋霉素的新方法.方法在碱性介质中乙酰螺旋霉素能强烈抑制luminol-K3Fe(CN)6化学发光反应.本文以乙酰螺旋霉素在一定浓度范围内与luminol-K3Fe(CN)6化学发光强度降低值的呈线性关系为基础,结合流动注射技术,快速测定乙酰螺旋霉素.结果测定的线性范围为0.1～100 μg·L-1,检测限为40 ng·L-1(3σ),RSD小于3.0%(n=8).结论该法简便快速、灵敏度高、选择性好,可用于药物、人血清中的乙酰螺旋霉素含量的测定,监测口服乙酰螺旋霉素后人尿液中乙酰螺旋霉素的排泄状况.
목적건립류동주사억제화학발광측정을선라선매소적신방법.방법재감성개질중을선라선매소능강렬억제luminol-K3Fe(CN)6화학발광반응.본문이을선라선매소재일정농도범위내여luminol-K3Fe(CN)6화학발광강도강저치적정선성관계위기출,결합류동주사기술,쾌속측정을선라선매소.결과측정적선성범위위0.1～100 μg·L-1,검측한위40 ng·L-1(3σ),RSD소우3.0%(n=8).결론해법간편쾌속、령민도고、선택성호,가용우약물、인혈청중적을선라선매소함량적측정,감측구복을선라선매소후인뇨액중을선라선매소적배설상황.
Aim To establish a new and simple flow injection method for the rapid determination of acetylspiramycin (ASPM). Methods ASPM was determined by chemiluminescence (CL) method combined with flow injection (FI) technology, which was based on the inhibitive effect of ASPM on the chemiluminescence reaction of the luminol-K3Fe(CN)6 system. Results The decrease of chemiluminescence intensity was proportional to the logarithm of ASPM concentration (0.1-100) μg·L-1, the detection limit was 40 ng·L-1 (3σ). The whole process, including sampling and washing, could be completed in 0.5 min with a RSD less than 3.0% (n=5). Conclusion The FI-CL method is of both high sensitivity and good selectivity giving a throughput of 120 h-1. The proposed method was applied successfully to the determination of ASPM in pharmaceutical preparations and human urine without any pre-treatment. It was found that the ASPM concentration reached its maximum after being orally administrated for two hours.