CAJ | 학술논문

目的观察糖皮质激素对骨髓基质细胞增殖及分化的作用,探讨糖皮质激素导致继发性骨质疏松的病理学机制.方法骨髓基质细胞以随机数字表法随机分为对照组和实验组.以 1× 10- 7 mol/L地塞米松加入髓基质细胞培养物中,测定增殖的骨髓基质细胞数及培养液中骨钙素含量.通过苏丹 III脂肪细胞染色计数观察地塞米松作用时间对脂肪细胞分化的影响.结果实验组及培养液中骨钙素含量 (5.2± 1.4) ng/mg蛋白质 ,明显低于对照组 (12.5± 1.5) ng/mg蛋白质 (t=12.61,P< 0.001);骨髓基质细胞数实验组 (3.73± 0.43)× 105个细胞 /孔 ,明显低于对照组 (5.76± 0.69)× 105个细胞 /孔 (t=7.45,P< 0.001);脂肪细胞的数量随地塞米松作用时间延长而增多.结论糖皮质激素抑制骨髓基质细胞增殖及向成骨细胞分化,促进其向脂肪细胞分化,这可能与糖皮质激素引起继发性骨质疏松时,骨量减少,髓内脂肪组织增多有关.
목적관찰당피질격소대골수기질세포증식급분화적작용,탐토당피질격소도치계발성골질소송적병이학궤제.방법골수기질세포이수궤수자표법수궤분위대조조화실험조.이 1× 10- 7 mol/L지새미송가입수기질세포배양물중,측정증식적골수기질세포수급배양액중골개소함량.통과소단 III지방세포염색계수관찰지새미송작용시간대지방세포분화적영향.결과실험조급배양액중골개소함량 (5.2± 1.4) ng/mg단백질 ,명현저우대조조 (12.5± 1.5) ng/mg단백질 (t=12.61,P< 0.001);골수기질세포수실험조 (3.73± 0.43)× 105개세포 /공 ,명현저우대조조 (5.76± 0.69)× 105개세포 /공 (t=7.45,P< 0.001);지방세포적수량수지새미송작용시간연장이증다.결론당피질격소억제골수기질세포증식급향성골세포분화,촉진기향지방세포분화,저가능여당피질격소인기계발성골질소송시,골량감소,수내지방조직증다유관.
Aim To observe the effect of glucocorticoids on marrow stromal cell proliferation and differentiation and study the pathological mechanism of osteoporosis.Methods Bone marrow matrix cells were divided into experiment and control groups according to random number table.Proliferation of marrow stromal cell treated with dexamethasone(1× 10- 7 mol/L) and contents of osteocalcin in the media were measured.Results The number of the marrow stromal cell[(3.73± 0.43)× 105]and the contents of osteocalcin[(5.2± 1.4)ng/mg]in the media of the experimental group obviously decreased compared to the control[(5.76± 0.69)× 105,(12.5± 1.5)ng/mg](t=12.61,7.45,P< 0.001).The number of adipocytes increased with longer duration of exposure to dexamethasone.Conclusion Glucocorticoids inhibited marrow stromal cell proliferation and osteoblastic differentiation, and promoted its differentiation into adipocytes,which is a major contributing factor of bone loss and an increase in marrow fat volume in the osteoporosis.