CAJ | 학술논문

【目的】探讨细胞周期调节因子在鼻咽癌复发中的作用。【方法】采用LsAB法同时检测69例患者原发和复发鼻咽癌组织中p53、MDM2、p21ras和p21WAF1蛋白的表达。【结果】复发癌与原发癌相比：阳性表达率方面，p53蛋白(78%和80%)或MDM2蛋白(84%和83%)很相近，p21ras蛋白(73%和93%)或p21WAF1蛋白(52%和84%)明显下降；高表达率方面，p53蛋白(42%和51%)很相近,MDM2蛋白(57%和32%)明显升高,p21ras蛋白(16%和65%)或p21WAF1蛋白(17%和46%)明显下降。其中，MDM2蛋白表达水平在复发癌明显升高主要见于复发间期<34个月患者组（P<0.05),p21ras蛋白和p21WAF1蛋白表达水平在复发癌明显下降见于复发间期<34个月和≥34个月患者组（均<0.02以下)。【结论】原发鼻咽癌临床治愈后，p53蛋白和MDM2蛋白过度表达以及p21WAF1蛋白低表达或不表达可能仍然对鼻咽癌的复发起重要作用；MDM2蛋白表达水平显著升高和p21WAF1蛋白表达水平显著下降可能进一步加促鼻咽癌的复发过程。
【목적】탐토세포주기조절인자재비인암복발중적작용。【방법】채용LsAB법동시검측69례환자원발화복발비인암조직중p53、MDM2、p21ras화p21WAF1단백적표체。【결과】복발암여원발암상비：양성표체솔방면，p53단백(78%화80%)혹MDM2단백(84%화83%)흔상근，p21ras단백(73%화93%)혹p21WAF1단백(52%화84%)명현하강；고표체솔방면，p53단백(42%화51%)흔상근,MDM2단백(57%화32%)명현승고,p21ras단백(16%화65%)혹p21WAF1단백(17%화46%)명현하강。기중，MDM2단백표체수평재복발암명현승고주요견우복발간기<34개월환자조（P<0.05),p21ras단백화p21WAF1단백표체수평재복발암명현하강견우복발간기<34개월화≥34개월환자조（균<0.02이하)。【결론】원발비인암림상치유후，p53단백화MDM2단백과도표체이급p21WAF1단백저표체혹불표체가능잉연대비인암적복발기중요작용；MDM2단백표체수평현저승고화p21WAF1단백표체수평현저하강가능진일보가촉비인암적복발과정。
【Objective】To explore the role of cell cycle regulators in nasopharyngeal carcinoma (NPC) relapse.【Method】To assay p53,MDM2,p21ras and p21WAF1 proteins by LsAB immunohistochemical technique in 69 cases of primary and relapsing NPC tissues.【Results】As compared with primary NPC,the expression rate of p53 or MDM2 protein in relapsing NPC was similar (78% to 80%,84% to 83%),and the expression rate of p21ras or p21WAF1 protein in relapsing NPC was obviously descended (73% to 93%,52% to 84% );the high-expression rate of p53 protein in relapsing NPC was similar (42% to 51%),the high-expression rate of MDM2 protein in relapsing NPC was obviously risen (57% to 32%),and the high-expression rate of p21ras or p21WAF1 protein in relapsing NPC was obviously descended (16% to 65%,17% to 46%).Among of them,the significant rise of MDM2 protein expression level in relapsing NPC mainly occurred in the patients of group 2 which relapsing-interval was shorter than 34 months,P<0.05;the significant descent of p21ras or p21WAF1 protein expression level in relapsing NPC occurred in the patients of group 2 and group 1 which relapsing-interval was equal to or longer than 34 months,P<0.02,respectively.【Conclusions】The overexpression of p53 and MDM2 proteins and the low or negative expression of p21WAF1 protein after clinical cure might still play an important role in NPC relapse,the obvious rise of MDM2 protein level and the obvious descent of p21WAF1 protein level might further accelarate the process of NPC relapse.