CAJ | 학술논문

采用目前公认的DNA氧化损伤标志物8-OHdG的单抗,利用LSAB法研究不同年龄组BALB/C小鼠胸腺和脾脏8-OHdG的生成水平,对免疫组织在衰老过程中DNA氧化损伤的水平进行免疫组织化学研究,以探讨免疫系统的自由基损伤对衰老的影响.结果表明,在胸腺,8-OHdG+细胞密度随增龄而增多,并主要位于髓质区;脾脏中的阳性细胞则无明显的增龄性变化,但胞染形态却存在明显差异.本研究结果显示,衰老过程中,免疫细胞内8-OHdG含量发生改变,免疫系统受到了氧自由基的损伤.8-OHdG可作为免疫老化过程中的一种生物标志.
채용목전공인적DNA양화손상표지물8-OHdG적단항,이용LSAB법연구불동년령조BALB/C소서흉선화비장8-OHdG적생성수평,대면역조직재쇠로과정중DNA양화손상적수평진행면역조직화학연구,이탐토면역계통적자유기손상대쇠로적영향.결과표명,재흉선,8-OHdG+세포밀도수증령이증다,병주요위우수질구;비장중적양성세포칙무명현적증령성변화,단포염형태각존재명현차이.본연구결과현시,쇠로과정중,면역세포내8-OHdG함량발생개변,면역계통수도료양자유기적손상.8-OHdG가작위면역노화과정중적일충생물표지.
To evaluate the levels of 8-Hydroxy-2'-deoxyguanosine (8-OHdG) in the lymphoid organs of different aged BALB/c mice. Methods An LSAB immunohistochemical method was used to detect the formation of 8-OHdG in the thymus and spleen of 1-, 5- and 10-month of BALB/c mice. Results The results showed that there was more extensive staining in the nuclei of thymic cells in the elder mice, and the 8-OHdG formation was mostly situated in medulla of thymic tissues. From cortex to medulla, positive cells were increased in number. In splenic tissues, the nucleus stainings were predominately localized in red pulp, but no visible age-related changes were observed both in the number and the density of the 8-OHdG+ spleen cells. In addition, the shapes of nucleus stainings appeared not the same among these spleen tissues with different ages. Conclusions The present results indicate that immune system may suffer from oxidative damage of endogenous and extrinsic oxygen free radicals in older mice, which could be reflected by the presence of 8-OHdG within immunocytes. Therefore, 8-OHdG could be a biomarker for DNA oxidative damage in immunosenescence.