CAJ | 학술논문

目的探讨新型高效有机磷农药水胺硫磷对人皮肤表皮细胞膜的影响。方法以体外浸泡式培养的人皮肤表皮细胞为生物材料，以培养基中乳酸脱氢酶（LDH）为指标，检测在不同染毒浓度条件下漏入培养基中的LDH活性，并同时检测培养基中碱性磷酸酶（ALP）的活性。结果随着染毒剂量的增加，培养基中LDH活性降低，与阳性及阴性对照组相比其差异显著(P＜0.05或P＜0.01)。各组培养基中的ALP活性无显著性差异(P＞0.05)。结论水胺硫磷对人皮肤表皮细胞无直接刺激损害，但对人皮肤表皮细胞中的LDH有抑制作用。本研究提示水胺硫磷和其它大部分已广泛使用的有机磷农药一样，可经完整的皮肤进入机体，在未发生皮肤损害时就可造成机体中毒，因此劳动防护十分重要。
목적탐토신형고효유궤린농약수알류린대인피부표피세포막적영향。방법이체외침포식배양적인피부표피세포위생물재료，이배양기중유산탈경매（LDH）위지표，검측재불동염독농도조건하루입배양기중적LDH활성，병동시검측배양기중감성린산매（ALP）적활성。결과수착염독제량적증가，배양기중LDH활성강저，여양성급음성대조조상비기차이현저(P＜0.05혹P＜0.01)。각조배양기중적ALP활성무현저성차이(P＞0.05)。결론수알류린대인피부표피세포무직접자격손해，단대인피부표피세포중적LDH유억제작용。본연구제시수알류린화기타대부분이엄범사용적유궤린농약일양，가경완정적피부진입궤체，재미발생피부손해시취가조성궤체중독，인차노동방호십분중요。
Objective　To investigate the effects of isocarbophos, a new and highly effective organophosphorus pesticide on the membranes of keratinocytes. Methods　The epidermic cells of human skin cultured in the medium were adopted as a biological material.The activity of lactate dehydrogenase (LDH) was used as an indicator. We tested the activities of LDH and alkaline phosphatase (ALP) in the medium in different isocarbophos dosage conditions. Results　It was found that the activity of LDH in the medium decreased with the increase of isocarbophos dosage. The LDH activities of the dosage groups were statistically different from those of the positive and negative groups. The ALP activities were of no significant difference between the groups. Conclusion　The above findings reveal that isocarbophos does not damage the membranes of keratinocytes directly, howerer it inhibits the LDH of human skin keratinocytes; thus it is suggested that isocarbophos like most organophosphorus pesticides used widely can pass through the intact human skin and poison the body before the skin is damaged. Therefore, protection during work hours is of paramount importance.