CAJ | 학술논문

目的：研究T细胞在巨噬细胞游走抑制因子（MIF）基因修饰的瘤苗诱导抗肿瘤免疫反应中的作用。方法：将重组MIF腺病毒转染小鼠FBL3红白血病细胞制成的瘤苗接种于小鼠体内，观察脾脏和淋巴结中T细胞数量和功能的改变。结果：经MIF基因修饰的瘤苗免疫后，小鼠脾细胞和腹股沟引流淋巴结细胞数量明显增多，脾细胞CTL活性显著增强，引流淋巴结中CD3＋、CD28＋、CD4＋和CD8＋ T细胞百分率均较对照组增加。MIF基因修饰的瘤苗免疫对小鼠受野生型FBL3肿瘤细胞再攻击具有明显的保护作用。结论：MIF基因修饰的瘤苗能诱导T细胞介导的抗肿瘤免疫反应，有可能作为肿瘤基因治疗的候选者。
목적：연구T세포재거서세포유주 억제인자（MIF）기인수식적류묘유도항종류면역반응중적작용。방법：장중조MIF선병독전염소서FBL3홍백혈병세포제성적류묘접충우소서체내，관찰비장화림 파결중T세포수량화공능적개변。결과：경MIF기인수식적류묘면역후 ，소서비세포화복고구인류림파결세포수량명현증다，비세포CTL활성현저증강，인류림파 결중CD3＋、CD28＋、CD4＋화CD8＋ T세포백분솔균교대조조증가。MIF기인수식적 류묘면역대소서수야생형FBL3종류세포재공격구유명현적보호작용。결론：MIF기인수식적류묘능유도T세포개도적항종류면역반응，유가능작위종류기인치료적 후선자。
Objective: To investigate the role of T cell in the antitumor immune responses induced by MIF gene-modified tumor vaccine. Methods: MIF gene was transferred into FBL3 erythroleukemia cel l by adenovirus carrier and a new type of tumor vaccine was prepared. The chang es of the number and the function of T cell in spleen and lymph node was observe d. Results: After the mice were immunized with MIF gene-m odified FBL3 vaccine, the number of lymphocyte in spleens and lymph nodes increa sed markedly and the specific CTL activities of splenocytes also increased great ly. FACS analysis showed that the CD3+,　CD4+,　CD8+ T cells and CD28 posi tive cells in draining lymph nodes of MIF-FBL3 group mice increased more marked ly than that of control groups. When the wild type FBL3 cells were injected into the mice immunized with MIF gene-modified FBL3 vaccine, the growth of tumors w ere obviously inhibited and the survival rate of the mice was increased. Conclusion: It is suggested that MIF gene-modified tumor vaccine can induce specific antitumor immune responses mediated by T cells and may be a candidate for gene therapy of tumor.