CAJ | 학술논문

目的研究新城疫病毒(NDV)核衣壳蛋白(NP)基因的生物学作用及其抗人喉鳞癌的作用。方法以提纯的NDVV4株基因组RNA为模板，化学合成NP基因的特异核苷酸引物，RT-PCR扩增NP基因cDNA，得到1条1.5kb的DNA带，与NDVNP基因大小一致，平端连接克隆到pUC119质粒中。结果阳性克隆经酶切鉴定及序列分析表明已获得NDV V4株NP基因克隆。结论将NDV V4株NP基因碱基序列与已发表的NDVBeaudetteC株、LaSota株、D26株的NP基因的碱基序列比较，同源性分别为90.64%、90.17%、98.03%，氨基酸序列差异率是4.50%、5.93%、2.45%。NDV V4株NP基因与已发表的NDVBeaudetteC株、LaSota株、D26株的NP基因有所不同，但具有高度同源性。
목적 연구신성역병독(NDV)핵의각단백(NP)기인적생물학작용급기항인후린암적작용。방법 이제순적NDVV4주기인조RNA위모판，화학합성NP기인적특이핵감산인물，RT-PCR확증NP기인cDNA，득도1조1.5kb적DNA대，여NDVNP기인대소일치，평단련접극륭도pUC119질립중。결과 양성극륭경매절감정급서렬분석표명이획득NDV V4주NP기인극륭。결론 장NDV V4주NP기인감기서렬여이발표적NDVBeaudetteC주、LaSota주、D26주적NP기인적감기서렬비교，동원성분별위90.64%、90.17%、98.03%，안기산서렬차이솔시4.50%、5.93%、2.45%。NDV V4주NP기인여이발표적NDVBeaudetteC주、LaSota주、D26주적NP기인유소불동，단구유고도동원성。
Objective To study biochemical function and therapy of theNP(nucleocapsid protein) gene of NDV(new castle disease virus).Methods The purified genome RNA of NDV were used for template.According to the reported NP gene sequence of NDV strain Beaudettec C,a pair of 19 mer primers was designed and synthesized.Then the NP gene of NDV strain V4 was amplified by reverse transcription-polymerase chain reaction(RT-PCR).The amplified products were analyzed by agarose gel electrophoresis,a specific fragment about 1.5 kb as expected appeared.The RT-PCR product of strain V4 was cloned into the pUC119,then the positive clone was tested by restriction endonuclease analysis and it was sequenced by Sanger's method.Results The result suggested that it was the NP gene of NDV strain V4.Conclusion The NP gene sequence of NDV V4 as compared with published NP gene sequence of NDV Beaudettec C,La Sota and D26,affinal are 90.64%,90.17%,98.03%;difference rate of the sequence of amino acid of the NP gene are 4.50%,5.93%,2.45%.