吉林农业大学学报
吉林農業大學學報
길임농업대학학보
JOURNAL OF JILIN AGRICUL TURAL UNIVERSITY
2001年
1期
40-45
,共6页
VA菌根真菌%烟草%山东%种的鉴定
VA菌根真菌%煙草%山東%種的鑒定
VA균근진균%연초%산동%충적감정
从山东烟区的不同类型的植烟土壤中,采集烟草根际土,经湿筛倾倒蔗糖浮选分离VA菌根真菌孢子,单孢接种烟苗在水培条件下,选出在烟草上能形成VA菌根的菌株进行分类研究。鉴定出烟草VA菌根真菌2个属11个种。属于球囊霉属Glomus的种:苏格兰球囊霉G.cale donium( Nicol.& Gerd.)Gerd.& Trappe,近明球囊霉 G.claroideum Schenck & Smith,缩球囊霉 G .constrictum Trappe,德里球囊霉 G.delhiense Mukerji, Bhattacharjee & T ewari,透光球囊霉 G.diaphanum Morton & Walker,幼套球囊霉 G.etunicatum Becker & Gerdmann, 聚集球囊霉G.glomerulatum Sieverding, 根内球囊霉G.intraradices Schenck & Smith,摩西球囊霉 G.mosseae (Nicol. & Gerd.) Gerd. & Trappe, 网状球囊霉G.retic ulatum Bhatta charjee & Mukerji;属于无梗孢囊霉属 Acaulospora的种:詹氏无梗孢囊霉 A.ger demanni i Schenck & Nicolson。其中德里球囊霉、聚集球囊霉、网状球囊霉和詹氏无梗孢囊霉4 个种为我国新纪录种。对各个种的形态特征进行了详细描述。
從山東煙區的不同類型的植煙土壤中,採集煙草根際土,經濕篩傾倒蔗糖浮選分離VA菌根真菌孢子,單孢接種煙苗在水培條件下,選齣在煙草上能形成VA菌根的菌株進行分類研究。鑒定齣煙草VA菌根真菌2箇屬11箇種。屬于毬囊黴屬Glomus的種:囌格蘭毬囊黴G.cale donium( Nicol.& Gerd.)Gerd.& Trappe,近明毬囊黴 G.claroideum Schenck & Smith,縮毬囊黴 G .constrictum Trappe,德裏毬囊黴 G.delhiense Mukerji, Bhattacharjee & T ewari,透光毬囊黴 G.diaphanum Morton & Walker,幼套毬囊黴 G.etunicatum Becker & Gerdmann, 聚集毬囊黴G.glomerulatum Sieverding, 根內毬囊黴G.intraradices Schenck & Smith,摩西毬囊黴 G.mosseae (Nicol. & Gerd.) Gerd. & Trappe, 網狀毬囊黴G.retic ulatum Bhatta charjee & Mukerji;屬于無梗孢囊黴屬 Acaulospora的種:詹氏無梗孢囊黴 A.ger demanni i Schenck & Nicolson。其中德裏毬囊黴、聚集毬囊黴、網狀毬囊黴和詹氏無梗孢囊黴4 箇種為我國新紀錄種。對各箇種的形態特徵進行瞭詳細描述。
종산동연구적불동류형적식연토양중,채집연초근제토,경습사경도자당부선분리VA균근진균포자,단포접충연묘재수배조건하,선출재연초상능형성VA균근적균주진행분류연구。감정출연초VA균근진균2개속11개충。속우구낭매속Glomus적충:소격란구낭매G.cale donium( Nicol.& Gerd.)Gerd.& Trappe,근명구낭매 G.claroideum Schenck & Smith,축구낭매 G .constrictum Trappe,덕리구낭매 G.delhiense Mukerji, Bhattacharjee & T ewari,투광구낭매 G.diaphanum Morton & Walker,유투구낭매 G.etunicatum Becker & Gerdmann, 취집구낭매G.glomerulatum Sieverding, 근내구낭매G.intraradices Schenck & Smith,마서구낭매 G.mosseae (Nicol. & Gerd.) Gerd. & Trappe, 망상구낭매G.retic ulatum Bhatta charjee & Mukerji;속우무경포낭매속 Acaulospora적충:첨씨무경포낭매 A.ger demanni i Schenck & Nicolson。기중덕리구낭매、취집구낭매、망상구낭매화첨씨무경포낭매4 개충위아국신기록충。대각개충적형태특정진행료상세묘술。
Chlamydospores and azygospores of vesicular arbuscular mycorr hizal fungi(VAMF) were isolated by wet sieving and decanting method from rhizosp herical soils of tobacco. Tobacco seedlings at four leaf stage were inoculated w ith single spores of fungal isolates and transferred into sterilized sand in bla ck plastic tubes and then cultured in solution with low level of nutrients. VAM on tobacco were reisolated and 11 species in 2 genera were indentified, includin g G. caledoniu, G.claroideum, G.constrictum, G.delhiense, G.diaphanum, G .etunicatum, G.glomerulatum, G.intraradices, G.mosseae and G.reticulat um i n genus Glomus, and A.gerdemannii in genus Acaulospora. Of which, G .delhiense ,G.glome-rulatum, G.reticulatum and A.gerdemannii were first recorded in China.