CAJ | 학술논문

目的研究溶剂汽油对大鼠皮肤真皮层细胞间质的影响。方法 45只Wistar雄性大鼠分为对照组、实验组和干预组，染毒方式为经皮染毒。实验组染毒剂量分别为：250mg/cm2×1 d(A组)，250mg/cm2×4d(B组)，250mg/cm2×8d(C组)，染毒面积为2cm×3cm。干预组则是涂抹防护剂后，再进行染毒，染毒剂量250mg/cm2×8d。染毒结束后，分离染毒部位皮肤，分别测定真皮层中胶原蛋白、弹性蛋白和糖胺聚糖的含量。结果 C组大鼠皮肤胶原蛋白含量明显低于对照组和干预组(P<0．05)；B组和C组的弹性蛋白含量低于对照组(P<0．05)，C组与干预组相比，也有统计学差异；各实验组和干预组糖胺聚糖含量均低于对照组(P<0．05或P<0．01)，但干预组糖胺聚糖含量高于C组(P<0．05)。结论溶剂汽油可降低染毒大鼠皮肤真皮层中胶原蛋白、弹性蛋白和糖胺聚糖的含量。
목적 연구용제기유대대서피부진피층세포간질적영향。방법 45지Wistar웅성대서분위대조조、실험조화간예조，염독방식위경피염독。실험조염독제량분별위：250mg/cm2×1 d(A조)，250mg/cm2×4d(B조)，250mg/cm2×8d(C조)，염독면적위2cm×3cm。간예조칙시도말방호제후，재진행염독，염독제량250mg/cm2×8d。염독결속후，분리염독부위피부，분별측정진피층중효원단백、탄성단백화당알취당적함량。결과 C조대서피부효원단백함량명현저우대조조화간예조(P<0．05)；B조화C조적탄성단백함량저우대조조(P<0．05)，C조여간예조상비，야유통계학차이；각실험조화간예조당알취당함량균저우대조조(P<0．05혹P<0．01)，단간예조당알취당함량고우C조(P<0．05)。결론 용제기유가강저염독대서피부진피층중효원단백、탄성단백화당알취당적함량。
Purpose To study the effect of gasoline on the extracellular matrix of dermis in rat. Methods 45 male Wistar rats were divided into control group, 3 different exposure level groups, which were exposed to gasoline on 2 cm× 3 cm skin area with the dose of 250 mg/cm2 for 1 (group A) ,4(group B)and 8 days (group C) respectively,and an intervention group exposed to 250 mg/cm2 × 8 days gasoline after the application of protective agent on skin. After the treatment, collagen, elastin and glycosaminoglycan (GAG) were assayed in skin departed from intoxicated area. Results Compared with control group, collagen was decreased in group C(P<0.05); elastin was decreased in group B and C (P<0.05); glycosaminoglycan was decreased in all exposure groups and intervention group ( P < 0.05 or P < 0.01 )。 Collagen, elastin and glycosaminoglycan(GAG) were significantly lower in group C than in intervention group ( P < 0.05 ). Conclusions The extracellular matrix of dermis,including collagen,elastin and GAG were decreased in rat dermal exposed to gasoline.