CAJ | 학술논문

为了获得血小板冰冻保护液(丙二醇/氯化钠/水,PG/NaCl/H2O)冰冻降温的数学模型,本研究使用差示扫描量热仪分别测定出不同浓度和不同比例条件下丙二醇/氯化钠/水三元溶液的凝固点(Tf),然后将已有的公式和实验所得的数据通过计算机进行拟合.结果获得了一个数学模型,该数学模型描述的是在PG/NaCl/H2O溶液中Tf变化与溶质浓度和PG/NaCl(质量比)两个参数变化之同的函数关系.结论:把该方程与血小板的渗透性特性的参数相结合,可以描述在平衡冷冻状态下,不同PG浓度时血小板的体积变化,指导血小板低温保存.
위료획득혈소판빙동보호액(병이순/록화납/수,PG/NaCl/H2O)빙동강온적수학모형,본연구사용차시소묘량열의분별측정출불동농도화불동비례조건하병이순/록화납/수삼원용액적응고점(Tf),연후장이유적공식화실험소득적수거통과계산궤진행의합.결과획득료일개수학모형,해수학모형묘술적시재PG/NaCl/H2O용액중Tf변화여용질농도화PG/NaCl(질량비)량개삼수변화지동적함수관계.결론:파해방정여혈소판적삼투성특성적삼수상결합,가이묘술재평형냉동상태하,불동PG농도시혈소판적체적변화,지도혈소판저온보존.
In order to acquire freezing model of the cryopretective solution (NaCl-propylene glycol-water ternary system)for platelet, the melting points (Tf) of this cryopretective solutions with different solute concentration and different ratio of PG mass to NaCl mass were measured by using a differential scanning calorimeter (DSC), and these experimental data were fitting by computer. An empirical equation was derived which characterized the Tf as a function of the solute concentration and the ratio of PG mass to NaCl mass inside this solution. It was concluded that the equilibrium freezing model for human platelets in this system could be used to instruct platelet cryopreserving techniques.