CAJ | 학술논문

目的：利用血管紧张素Ⅱ受体拮抗药洛沙坦、PD123319研究血管紧张素Ⅱ2型受体对培养的大鼠主动脉平滑肌细胞增殖的影响。方法：将含有血管紧张素Ⅱ2型受体cDNA的质粒转染入培养的大鼠主动脉平滑肌细胞后，实验组分为血管紧张素Ⅱ处理组(组1)、血管紧张素Ⅱ和洛沙坦处理组(组2)、血管紧张素Ⅱ和PD123319处理组(组3)。检测核增殖抗原表达、细胞数目及一氧化氮合酶含量的变化。结果：组2细胞数目为(4.17±0.15)×105个，核增殖抗原平均光密度为0.202 6±0.007 6，较组3细胞明显减少(P＜0.01)；而组2细胞一氧化氮合酶平均光密度为0.027 5±0.002 1，明显高于组3细胞(0.016 9±0.002 0)(P＜0.01)。结论：血管紧张素Ⅱ可能通过其2型受体表现为抑制血管平滑肌细胞增殖、拮抗1型受体的作用，血管紧张素Ⅱ2型受体的这种作用可能与增强一氧化氮合酶活性，使细胞合成、释放一氧化氮增多有关。
목적：이용혈관긴장소Ⅱ수체길항약락사탄、PD123319연구혈관긴장소Ⅱ2형수체대배양적대서주동맥평활기세포증식적영향。방법：장함유혈관긴장소Ⅱ2형수체cDNA적질립전염입배양적대서주동맥평활기세포후，실험조분위혈관긴장소Ⅱ처리조(조1)、혈관긴장소Ⅱ화락사탄처리조(조2)、혈관긴장소Ⅱ화PD123319처리조(조3)。검측핵증식항원표체、세포수목급일양화담합매함량적변화。결과：조2세포수목위(4.17±0.15)×105개，핵증식항원평균광밀도위0.202 6±0.007 6，교조3세포명현감소(P＜0.01)；이조2세포일양화담합매평균광밀도위0.027 5±0.002 1，명현고우조3세포(0.016 9±0.002 0)(P＜0.01)。결론：혈관긴장소Ⅱ가능통과기2형수체표현위억제혈관평활기세포증식、길항1형수체적작용，혈관긴장소Ⅱ2형수체적저충작용가능여증강일양화담합매활성，사세포합성、석방일양화담증다유관。
Objective:To observe the affection of angiotensin Ⅱ antagonists on the cultured subtype 2 receptor of angiotensin II transfected aortic vascular smooth muscle cells of rat.Methods:After transfected the plasmid that contained the cDNA of subtype 2 receptor of angiotensin II into cultured rat vascular smooth muscle cells, the cells were divided into three groups：cells of group 1 were treated with angiotensinⅡ,cells of group 2 were treated with angiotensinⅡand losartan,cells of group 3 were treated with angiotensinⅡ and PD123319 .After experiments，the expression of PCNA, NOS and the cell number was tested, respectively.Results：After treated with Losartan，the cell number of group 2 was(4.17±0.15)×105，the OD value of PCNA was 0.202 6±0.007 6，both of which were less than that of cells of group 3；the OD value of NOS of cells was more in group 2(0.027 5±0.002 1 ) than that in group 3 (0.016 9±0.002 0) (P＜0.01).Conclusions:It suggests that when being activated,subtype 2 receptor of angiotensin Ⅱ could inhibit the proliferation of vascular smooth muscle cells and antagonist the effect of subtype 1 receptor of angiotensin Ⅱ,such an effect may be related to the activation of NOS.