CAJ | 학술논문

利用C分带、基因组原位杂交并结合分子生物学手段,对12份巨穗小麦种质材料中的外源遗传物质进行了检测.结果表明,12份材料染色体数均为42,其中5份材料均具有一对小麦-黑麦(Triticum aestivum-Secalecereal)1BL/1RS易位染色体和一对中间偃麦草(Agropyron intermedium Garten)染色体、3份材料只具有一对中间偃麦草染色体、3份材料只具一对1BL/1RS染色体、1份材料无1BL/1RS和中间偃麦草染色体.进一步细胞学分析表明,此中间偃麦草染色体代换了普通小麦(Triticum aestivum L.)中的2D染色体,因其良好的同源补偿性,表示为2Ai.同时对2Ai在巨穗小麦种质中存在的遗传学意义及小麦遗传改良中的应用进行了讨论.
이용C분대、기인조원위잡교병결합분자생물학수단,대12빈거수소맥충질재료중적외원유전물질진행료검측.결과표명,12빈재료염색체수균위42,기중5빈재료균구유일대소맥-흑맥(Triticum aestivum-Secalecereal)1BL/1RS역위염색체화일대중간언맥초(Agropyron intermedium Garten)염색체、3빈재료지구유일대중간언맥초염색체、3빈재료지구일대1BL/1RS염색체、1빈재료무1BL/1RS화중간언맥초염색체.진일보세포학분석표명,차중간언맥초염색체대환료보통소맥(Triticum aestivum L.)중적2D염색체,인기량호적동원보상성,표시위2Ai.동시대2Ai재거수소맥충질중존재적유전학의의급소맥유전개량중적응용진행료토론.
Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding,genomicin situ hybridization (GISH), sequence characterized amplified region (SCAR), and random ampli-fied polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried botha pair of wheat-rye (7riticum aestivum-Secale cereal) 1BL/1 RS translocation chromosomes and a pair ofAgropyronintermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three otherscarried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome.Further identification revealed that the identical Ai chromosome in these germplasm lines substituted thechromosome 2D of common wheat (T. aestivum L.), designated as 2Ai. The genetic implication and furtherutilization of 2Ai in wheat improvement were also discussed.