CAJ | 학술논문

目的探讨一种新型微孔底膜插入式培养皿的制作方法, 为进行体外细胞共培养提供技术支持.方法利用塑料离心管, 微孔滤膜为材料制备微孔底膜插入式培养皿; 在该插入式培养皿中接种大鼠脑微血管内皮细胞 (brain capillary endothelial cells, BCECs)(在星形胶质细胞条件培养液影响下), 以跨内皮细胞阻抗(trans-endothelial electronic resistance, TEER)为指标对该插入式培养皿进行评价.结果该插入式培养皿在培养条件下(未接种细胞)15 天内未出现破损; 共培养条件下TEER增幅明显:由(66.1±13.3) Ωcm2升至(182.2±6.7) Ωcm2.结论该微孔底膜插入式培养皿制作简便, 脑微血管内皮细胞在上生长良好, 并具有可调节性和可重复使用的特点, 是一种可用于体外细胞共培养相关研究工作有效的工具.
목적탐토일충신형미공저막삽입식배양명적제작방법, 위진행체외세포공배양제공기술지지.방법이용소료리심관, 미공려막위재료제비미공저막삽입식배양명; 재해삽입식배양명중접충대서뇌미혈관내피세포 (brain capillary endothelial cells, BCECs)(재성형효질세포조건배양액영향하), 이과내피세포조항(trans-endothelial electronic resistance, TEER)위지표대해삽입식배양명진행평개.결과해삽입식배양명재배양조건하(미접충세포)15 천내미출현파손; 공배양조건하TEER증폭명현:유(66.1±13.3) Ωcm2승지(182.2±6.7) Ωcm2.결론해미공저막삽입식배양명제작간편, 뇌미혈관내피세포재상생장량호, 병구유가조절성화가중복사용적특점, 시일충가용우체외세포공배양상관연구공작유효적공구.
Aim To prepare a hand-made micropore membrane culture plate insert for co-culture. Methods The plate insert was made using plastic centrifuge tube and micropore membrane. After seeding brain capillary endothelial cells (BCECs) on it (under the effect of astrocyte-conditioned medium), the plate insert was assessed by analysis of trans-endothelial electrical resistance (TEER). Results The plate insert has a stability of at least 15 d under culture condition. TEER increased significantly under co-culture condition from (66.1±13.3) Ωcm2 to (182.2±6.7) Ωcm2. Conclusion This micropore membrane culture plate insert can be easily made, on which BCEC culture can be successfully performed. Moreover, it is adjustable and recyclable. It follows that the plate insert is a useful tool for co-culture and the related research fields.