CAJ | 학술논문

利用拮抗芽孢杆菌菌株B96-Ⅱ对芦笋茎枯病菌进行了室内抑菌试验和田间防治及机理研究,结果表明:B96-Ⅱ对芦笋茎枯病菌有明显抑制作用.稀释50～1600倍后平皿试验,生长速率抑制率为97.37%～92.98%;离体组织培养对菌斑的抑制率为94.40%;田间防治效果为93.40%.显微观察发现,B96-Ⅱ处理后可导致菌丝和孢子严重破损,使细胞内容物外渗.处理后24h,菌液电导率(μs·cm~(-1))和总溶解固体(TDS)比不处理的对照明显提高,处理中B96-Ⅱ培养液浓度从1%升到10%时,电导率增加量从47.50%提高到518.33%.总溶解固体增加量从176.10%提高到797.60%.经测定,B96-Ⅱ可抑制病原菌菌丝呼吸,处理中B96-Ⅱ培养液浓度从1%升到10%时呼吸抑制率从25.00%升至196.40%.生物测定表明,B96-Ⅱ的主要抑菌物为拮抗蛋白.
이용길항아포간균균주B96-Ⅱ대호순경고병균진행료실내억균시험화전간방치급궤리연구,결과표명:B96-Ⅱ대호순경고병균유명현억제작용.희석50～1600배후평명시험,생장속솔억제솔위97.37%～92.98%;리체조직배양대균반적억제솔위94.40%;전간방치효과위93.40%.현미관찰발현,B96-Ⅱ처리후가도치균사화포자엄중파손,사세포내용물외삼.처리후24h,균액전도솔(μs·cm~(-1))화총용해고체(TDS)비불처리적대조명현제고,처리중B96-Ⅱ배양액농도종1%승도10%시,전도솔증가량종47.50%제고도518.33%.총용해고체증가량종176.10%제고도797.60%.경측정,B96-Ⅱ가억제병원균균사호흡,처리중B96-Ⅱ배양액농도종1%승도10%시호흡억제솔종25.00%승지196.40%.생물측정표명,B96-Ⅱ적주요억균물위길항단백.
The inhibition effects of an antagonistic Bacillus strain B96-II on asparagus stem blight (Phomopsis asparagi Sacc.) were investigated under laboratory in vitro and field conditions. The results demonstrate that B96-II effectively inhibits the pathogen. Inhabitation rates on P. asparagi growth rate are between 97.37% and 92.98%, when the cultures treated with 50 to 1 600 times dilution of B96-II fermentation brew. Under in vitro conditions, colonial growth on asparagus stem reduces by 94.40% compared with the control samples, and under field trials by 93.40%. Microscopic observation reveals that the mycelia and spore walls of P. asparagi are destroyed and cell contents released after B96-II treatment. Conductivity of the inoculum liquid of P. asparagi increases significantly 24 h after treatments. As B96-II inoculum liquid concentration increases from 1% to 10%, its conductivity increment increases from 47.50% to 518.33% and total dissolved solids (TDS) increment increases from 176.10% to 797.60%. B96-II also restricts P. asparagi mycelial respiration at an inhibition rate of 25.00% by addition of 1% B96-II and 196.40% by addition of 10% B96-II. Based on the analysis, the main active substances in B96-II are antagonistic proteins.