CAJ | 학술논문

从北京市农林科学院筛选出的100对玉米SSR核心引物中选取20对,利用SSR分子标记技术,构建了贵州省2000年以来审定的48个玉米杂交种及其亲本的数字化指纹图谱.图谱构建所用的20个SSR标记每个位点的等位基因数平均为5.3个,多态信息量平均为0.66,标记索引系数平均为3.58,多态性丰富.图谱中,来自不同地区或不同育种家的同名自交系,有的指纹图谱较一致,有的有差异,有的扩增片段大小不同,有的扩增片段数目也不同;杂交种的谱带表现为偏母型、偏父型、杂种型和双亲互补型4种类型.经验证,建立图谱时所用的材料符合最小品种内变异的原则,表明所建立的图谱是准确的.该图谱对于玉米品种及其亲本的纯度鉴定、真实性鉴定以及维护育种家的权益有一定的应用价值.
종북경시농림과학원사선출적100대옥미SSR핵심인물중선취20대,이용SSR분자표기기술,구건료귀주성2000년이래심정적48개옥미잡교충급기친본적수자화지문도보.도보구건소용적20개SSR표기매개위점적등위기인수평균위5.3개,다태신식량평균위0.66,표기색인계수평균위3.58,다태성봉부.도보중,래자불동지구혹불동육충가적동명자교계,유적지문도보교일치,유적유차이,유적확증편단대소불동,유적확증편단수목야불동;잡교충적보대표현위편모형、편부형、잡충형화쌍친호보형4충류형.경험증,건립도보시소용적재료부합최소품충내변이적원칙,표명소건립적도보시준학적.해도보대우옥미품충급기친본적순도감정、진실성감정이급유호육충가적권익유일정적응용개치.
Twenty pairs of SSR primers from 100 pairs of core SSR maize primers screened by Beijing Academy of Agricultural and Forestry Sciences were selected to establish SSR fingerprint mapping of 48 hybrid maize varieties and their parents approved by Guizhou Crops Variety Examination Committee since 2000. The average alleles,polymorphic information content and marker index coefficient of 20 SSR markers are 5.3, 0.66 and 3.58 respectively.There is the same or different fingerprint mapping and different size or amount of amplified fragments in the fingerprint mapping of the same name inbred lines from different regions or different breeders. There are 4 types (matroclinous type, partial paternal type, hybrid type and two-parent complementary type) in bands of hybrid varieties. The used materials for mapping establishment accords with the principle of variation in minimum varieties, which indicates the established mapping is correct. The mapping has the application value in purity and reality identification of maize varieties and their parents, and protection of breeders' rights.