CAJ | 학술논문

为了解谷氨酰胺合成酶(Glutamine synthetase,GS)同工酶在小麦中的种类、活性及亚基组成状况,利用盐析、凝胶过滤、阴离子交换层析等技术对小麦苗期叶片中的GS同工酶进行了分离纯化及鉴定.通过测定GS活性跟踪GS的纯化过程,利用Native-PAGE结合活性染色技术检测GS的纯化效果,利用SDS-PAGE鉴定GS的亚基组成.结果表明,在小麦幼苗叶片中检测到3种GS同工酶GS1、GS2和GSx,其中Gsx含量少,但活性高.从小麦幼苗叶片中纯化得到2个GS同工酶即GS1和GS2.GS1由39 kD的亚基组成,GS2由45 kD的亚基组成,没有纯化得到GSx.
위료해곡안선알합성매(Glutamine synthetase,GS)동공매재소맥중적충류、활성급아기조성상황,이용염석、응효과려、음리자교환층석등기술대소맥묘기협편중적GS동공매진행료분리순화급감정.통과측정GS활성근종GS적순화과정,이용Native-PAGE결합활성염색기술검측GS적순화효과,이용SDS-PAGE감정GS적아기조성.결과표명,재소맥유묘협편중검측도3충GS동공매GS1、GS2화GSx,기중Gsx함량소,단활성고.종소맥유묘협편중순화득도2개GS동공매즉GS1화GS2.GS1유39 kD적아기조성,GS2유45 kD적아기조성,몰유순화득도GSx.
To confirm the role of glutamine synthetase isoforms in the nitrogen assimilation of wheat, three glutamine synthetase isoforms, GS1, GS2 and GSx in the wheat leaf during seedling period were purified with salting-out, gel filtration chromatography and anion-exchange chromatography. The purification process was traced by determining the GS activity of each fraction, and the purification effect was examined by Native-PAGE combined with activity staining. The subunit component of GS isoforms was analyzed by SDS-PAGE. The result showed that GS1 and GS2 was purified from leaves of wheat seedlings. GS1 was composed with a 39 kD subunit, and GS2 was composed with a 45 kD subunit. GSx was not obtained by this purification process.