CAJ | 학술논문

背景:补肾中药为骨伤科常用药,但对不同有效成分用于骨愈合的比较研究尚缺乏.目的:观察不同补肾中药有效成分对骨损伤大鼠骨愈合及血液流变学的影响.方法:建立大鼠股骨干骨折模型并分别用提取的骨碎补总黄酮、淫羊藿总黄酮、菟丝子总黄酮、柚皮甙、槲皮素,以及橙皮甙对造模大鼠进行灌胃治疗.21 d 后取血及骨标本,检测大鼠骨愈合程度和血液流变学指标.结果与结论:不同补肾中药有效成分均对骨愈合有益,且骨碎补总黄酮、淫羊藿总黄酮效果优于其他4 种药物.骨碎补总黄酮和淫羊藿总黄酮可有效抑制低剪切速下大鼠的血液黏度,降低红细胞聚集指数及血小板聚集性和黏附性(P < 0.05),但对红细胞变形性无显著作用.说明补肾中药有效成分可促进骨损伤的愈合,且对血液流变学有一定作用,以骨碎补总黄酮效果最佳.
배경:보신중약위골상과상용약,단대불동유효성분용우골유합적비교연구상결핍.목적:관찰불동보신중약유효성분대골손상대서골유합급혈액류변학적영향.방법:건립대서고골간골절모형병분별용제취적골쇄보총황동、음양곽총황동、토사자총황동、유피대、곡피소,이급등피대대조모대서진행관위치료.21 d 후취혈급골표본,검측대서골유합정도화혈액류변학지표.결과여결론:불동보신중약유효성분균대골유합유익,차골쇄보총황동、음양곽총황동효과우우기타4 충약물.골쇄보총황동화음양곽총황동가유효억제저전절속하대서적혈액점도,강저홍세포취집지수급혈소판취집성화점부성(P < 0.05),단대홍세포변형성무현저작용.설명보신중약유효성분가촉진골손상적유합,차대혈액류변학유일정작용,이골쇄보총황동효과최가.
BACKGROUND: Chinese kidney-tonifying herb is commonly used in orthopedics department. However, individual effects of different ingredients remain poorly understood. OBJECTIVE: To observe influence of effective ingredients of Chinese kidney-tonifying drugs on bone healing and hemorheology of rats with bone injury. METHODS: Rat model of fracture of shaft of the femur was established, and treated by intragastric administration separately with extractive rhizoma drynariae total flavonoids, epimedium total flavonoids, dodder total flavonoids, naringin, quercetin and hesperidin. Blood and bone samples were harvested after 21 days to detect the extent of bone healing and hemorheology indexes. RESULTS AND CONCLUSION: The effective constituents of different Chinese kidney-tonifying drugs were beneficial to bone healing, and the effect of rhizoma drynariae total flavonoids and epimedium total flavonoids was better than the other drugs. Rhizoma drynariae total flavonoids and epimedium total flavonoids effectively inhibited blood viscosity under low shear, and reduced erythrocyte aggregation index and the aggregation and adhesion of platelet (P < 0.05), but it had no evident effects on erythrocyte deformation. This shows that the effective ingredient of the Chinese kidney-tonifying drugs can promote bone healing and exert certain effects on hemorheology. In particular, rhizoma drynariae total flavonoids exhibits the best effect.