CAJ | 학술논문

目的探讨星形细胞肿瘤染色体10q杂合性缺失与其病理特征、发生发展及预后的关系.方法应用间期荧光原位杂交(FISH)技术检测85例星形细胞肿瘤组织(WHOⅡ级35例,WHOⅣ级50例)中染色体10q的杂合性缺失情况.结果 (1)35例弥漫型星形细胞瘤(WHOⅡ级)中,22例(62.9％)无异常改变,6例(17.1％)染色体10q杂合性缺失,7例(20.0％) 10号染色体多体.50例胶质母细胞(WHOⅣ级)中,5例(10.0％)无异常改变,34例(68.0％)染色体10q杂合性缺失,11例(22.0％)10号染色体多体.(2)染色体10q在低年龄组的缺失率为36.4％(12/33),高年龄组的缺失率为82.4％ (28/34),二者差异有统计学意义(P＜0.05);在弥漫型星形细胞瘤的缺失率为21.4％ (6/28),胶质母细胞瘤的缺失率为87.2％(34/39),二者差异有统计学意义(P＜0.05).单因素生存分析结果显示,年龄、肿瘤级别、染色体10q杂合性缺失与星形细胞肿瘤患者术后生存时间相关(P＜0.05).结论星形细胞肿瘤染色体10q杂合性缺失与患者的年龄、肿瘤级别有关.10q杂合性缺失与星形细胞肿瘤的进展有关,对星形细胞肿瘤患者的预后评估具有一定的价值.
목적 탐토성형세포종류염색체10q잡합성결실여기병리특정、발생발전급예후적관계.방법 응용간기형광원위잡교(FISH)기술검측85례성형세포종류조직(WHOⅡ급35례,WHOⅣ급50례)중염색체10q적잡합성결실정황.결과 (1)35례미만형성형세포류(WHOⅡ급)중,22례(62.9％)무이상개변,6례(17.1％)염색체10q잡합성결실,7례(20.0％) 10호염색체다체.50례효질모세포(WHOⅣ급)중,5례(10.0％)무이상개변,34례(68.0％)염색체10q잡합성결실,11례(22.0％)10호염색체다체.(2)염색체10q재저년령조적결실솔위36.4％(12/33),고년령조적결실솔위82.4％ (28/34),이자차이유통계학의의(P＜0.05);재미만형성형세포류적결실솔위21.4％ (6/28),효질모세포류적결실솔위87.2％(34/39),이자차이유통계학의의(P＜0.05).단인소생존분석결과현시,년령、종류급별、염색체10q잡합성결실여성형세포종류환자술후생존시간상관(P＜0.05).결론 성형세포종류염색체10q잡합성결실여환자적년령、종류급별유관.10q잡합성결실여성형세포종류적진전유관,대성형세포종류환자적예후평고구유일정적개치.
Objective To study the correlation between loss of heterozygosity (LOH) on chromosome 10q and pathologic features,pathogenesis,prognosis of astrocytic tumors.Methods LOH on 10q was studied by interphase fluorescence in-situ hibridization (FISH) in 85 cases of astrocytic tumor,including 35 cases of WHO grade Ⅱ tumors and 50 cases of WHO grade Ⅳ tumors.Results LOH on 10q was detected in 6 cases (17.1％) of diffuse astrocytoma (WHO grade H) and 34 cases (68.0％) of glioblastoma (WHO grade Ⅳ).10q polysomy was detected in 7 cases (20.0％) of diffuse astrocytoma and 11 cases (22.0％) of glioblastoma.The rates of LOH on 10q in young age group and elderly group were 36.4％ (12/33) and 82.4％ (28/34),respectively.The difference was of statistical significance (P ＜0.05).The rates of LOH on 10q in the diffuse astroeytoma and glioblastoma were 21.4％ (6/28) and 87.2％ (34/39),respeetively.The difference was also statistically significant (P ＜ 0.05).Univariate survival analysis showed that patient age,pathologic grade and 10q on LOH correlated with duration of survival (P ＜0.05).Conclusions There are correlation between 10q LOH,patient age and pathologic grade of astrocytic tumors.LOH on 10q is also related to the pathogenesis of astrocytic tumors and is helpful in predieting prognosis.