CAJ | 학술논문

目的探讨β神经生长因子(β-NGF)及其受体[酪氨酸激酶A(tyrosine kinase A,TrKA)和P75NGFR]在人胰腺导管癌组织中的表达及意义.方法选择胰腺导管癌组织标本80例和正常胰腺组织20例,采用免疫组化、荧光定量PCR等方法,定量检测β-NGF及其受体表达,分析β-NGF)及其受体与临床病理学特征,尤其与神经侵袭的关系.结果随着癌细胞分化程度降低和肿瘤TNM分期增加,β-NGF、TrKA蛋白表达水平增加,且低分化与高分化、中分化组相比显著增加(P<0.01),Ⅲ～Ⅳ期较Ⅰ～Ⅱ期显著增加(P<0.05);β-NGF、TrKA蛋白表达水平在有神经浸润组明显高于无神经浸润组(P<0.01),有淋巴结转移组明显高于无淋巴结转移组(P<0.01),且邻近神经组织的肿瘤细胞β-NGF、TrKA阳性程度高于远离神经组织的癌细胞.P75NGFR表达与肿瘤细胞分化程度关系密切.β-NGF和TrKA表达呈正相关.β-NGF、TrKA和P75NGFR mRNA表达水平在胰腺癌组织中明显高于正常胰腺组织,分别增加3.84、4.23和2.41倍.结论 β-NGF及其受体参与介导胰腺癌的发生,与胰腺癌临床病理学特征关系密切,β-NGF、TrKA高表达和胰腺癌嗜神经性关系密切.
목적 탐토β신경생장인자(β-NGF)급기수체[락안산격매A(tyrosine kinase A,TrKA)화P75NGFR]재인이선도관암조직중적표체급의의.방법 선택이선도관암조직표본80례화정상이선조직20례,채용면역조화、형광정량PCR등방법,정량검측β-NGF급기수체표체,분석β-NGF)급기수체여림상병이학특정,우기여신경침습적관계.결과 수착암세포분화정도강저화종류TNM분기증가,β-NGF、TrKA단백표체수평증가,차저분화여고분화、중분화조상비현저증가(P<0.01),Ⅲ～Ⅳ기교Ⅰ～Ⅱ기현저증가(P<0.05);β-NGF、TrKA단백표체수평재유신경침윤조명현고우무신경침윤조(P<0.01),유림파결전이조명현고우무림파결전이조(P<0.01),차린근신경조직적종류세포β-NGF、TrKA양성정도고우원리신경조직적암세포.P75NGFR표체여종류세포분화정도관계밀절.β-NGF화TrKA표체정정상관.β-NGF、TrKA화P75NGFR mRNA표체수평재이선암조직중명현고우정상이선조직,분별증가3.84、4.23화2.41배.결론 β-NGF급기수체삼여개도이선암적발생,여이선암림상병이학특정관계밀절,β-NGF、TrKA고표체화이선암기신경성관계밀절.
Objective To investigate nerve growth factor (β-NGF) and its receptors expression in human pancreatic ductal adenocarcinoma. Methods Expression and distribution of β-NGF, tyrosine kinase A (TrKA) and P75NGFR were detected in operation tissue specimens of pancreatic ductal adenocarcinoma with immunohistochemistry and real-time PCR. Relations of β-NGF and its receptors with clinicalpathological characters, especially nerve invasion were analyzed. Results β-NGF and TrKA expression are higher in pancreatic adenocarcinoma than normal pancreas, and the differences are significant (P < 0. 01). Β-NGF and TrKA expression are associated with the differentiation grades(DG), lymphatic node metastasis, nerve invasion and surgical pathological stages. Poorer of DG and later stages, more expression of β-NGF and TrKA. Β-NGF and TrKA expression have positive correlations. Β-NGF, TrKA and P75NGFR mRNA expression have significantly increased 3.84,4. 23 and 2. 41 times than normal tissues by real-time PCR, respectively. Conclusions β-NGF and TrKA might play potential rules in carcinogenesis for pancreatic cancer,have affinity with clinicopathological characters of pancreatic cancer. Β-NGF and TrKA may have mutual effect in signal transduction leading to perineural invasion of pancreatic carcinoma.