中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2010年
3期
253-257
,共5页
Humanin%Aβ_(1-42)%Morris水迷宫%阿尔茨海默病%微管相关蛋白2%Synapsin 1
Humanin%Aβ_(1-42)%Morris水迷宮%阿爾茨海默病%微管相關蛋白2%Synapsin 1
Humanin%Aβ_(1-42)%Morris수미궁%아이자해묵병%미관상관단백2%Synapsin 1
Humanin%Aβ_(1-42)%Morris water maze%Alzheimer's disease%Microtubule associated protein 2%Synapsin 1
目的 探讨Humanin(HN)对Aβ_(1-42)所致阿尔茨海默病(AD)大鼠空间记忆的影响及其作用机制.方法 36只Wistar大鼠按照随机数字表法分为生理盐水组(海马注射生理盐水)、AD模型组(海马注射Aβ_(1-42))和HN治疗组(海马注射Aβ_(1-42)制成AD模型后在同一部位注入2 μL HN),每组各12只.对各组大鼠进行Morris水迷宫空间记忆能力测试.免疫组织化学方法检测各组大鼠微管相关蛋白2(MAP2)的表达.利用Western blot方法检测各组大鼠synapsin 1蛋白水平.结果 与生理盐水组相比,AD模型组Morris水迷宫测试的潜伏期明显延长,MAP2表达明显降低,差异均有统计学意义(P<0.05),HN组则无统计学意义(P>0.05).Synapsin 1蛋白在HN治疗组中表达明显高于生理盐水组和AD模型组,差异均有统计学意义(P<0.05),而生理盐水组和AD模型组之间差异无统计学意义(P>0.05).结论 HN可以显著改善由Aβ_(1-42)所致AD大鼠的空间记忆能力,推测与HN提高MAP2与synapsin 1蛋白表达有关.
目的 探討Humanin(HN)對Aβ_(1-42)所緻阿爾茨海默病(AD)大鼠空間記憶的影響及其作用機製.方法 36隻Wistar大鼠按照隨機數字錶法分為生理鹽水組(海馬註射生理鹽水)、AD模型組(海馬註射Aβ_(1-42))和HN治療組(海馬註射Aβ_(1-42)製成AD模型後在同一部位註入2 μL HN),每組各12隻.對各組大鼠進行Morris水迷宮空間記憶能力測試.免疫組織化學方法檢測各組大鼠微管相關蛋白2(MAP2)的錶達.利用Western blot方法檢測各組大鼠synapsin 1蛋白水平.結果 與生理鹽水組相比,AD模型組Morris水迷宮測試的潛伏期明顯延長,MAP2錶達明顯降低,差異均有統計學意義(P<0.05),HN組則無統計學意義(P>0.05).Synapsin 1蛋白在HN治療組中錶達明顯高于生理鹽水組和AD模型組,差異均有統計學意義(P<0.05),而生理鹽水組和AD模型組之間差異無統計學意義(P>0.05).結論 HN可以顯著改善由Aβ_(1-42)所緻AD大鼠的空間記憶能力,推測與HN提高MAP2與synapsin 1蛋白錶達有關.
목적 탐토Humanin(HN)대Aβ_(1-42)소치아이자해묵병(AD)대서공간기억적영향급기작용궤제.방법 36지Wistar대서안조수궤수자표법분위생리염수조(해마주사생리염수)、AD모형조(해마주사Aβ_(1-42))화HN치료조(해마주사Aβ_(1-42)제성AD모형후재동일부위주입2 μL HN),매조각12지.대각조대서진행Morris수미궁공간기억능력측시.면역조직화학방법검측각조대서미관상관단백2(MAP2)적표체.이용Western blot방법검측각조대서synapsin 1단백수평.결과 여생리염수조상비,AD모형조Morris수미궁측시적잠복기명현연장,MAP2표체명현강저,차이균유통계학의의(P<0.05),HN조칙무통계학의의(P>0.05).Synapsin 1단백재HN치료조중표체명현고우생리염수조화AD모형조,차이균유통계학의의(P<0.05),이생리염수조화AD모형조지간차이무통계학의의(P>0.05).결론 HN가이현저개선유Aβ_(1-42)소치AD대서적공간기억능력,추측여HN제고MAP2여synapsin 1단백표체유관.
Objective To explore the effects of Humanin(HN)on the spatial memory and its mechanism in rats with Aβ_(1-42)-induced Alzheimer's disease.Methods Thirty-six Wistar rats were randomly divided into 3 groups:saline group,AD model group and HN-treatment group.The AD models were established by injection of Aβ_(1-42) into the hippocampus.HN-treatment group was given 2 μL HN at the same site after the success of model making.The spatial memory ability of the rats was tested with Morris water maze.Immunohistochemistry was used to detect the expression of microtubule associated protein 2(MAP2).The protein level of synapsin 1 was detected by Western blotting.Results AD model group showed significantly longer latency and decreased expression of MAP2 than that of the saline group(P<0.05);however,no significant differences of the latency and expression of MAP2 were found between the AD model group and the HN-treatment group(P>0.05).The expression of synapsin 1 in the HN-treatment group was significantly higher than that in the saline and the AD model groups(P<0.05);while no significant differences between the saline and AD model groups were found(P>0.05).Conclusion HN can improve the spatial memory of rats with Aβ_(1-42)-induced AD through iucreasing the expression of MAP2 and sympsin 1.
