中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2008年
7期
499-501
,共3页
张严峻%徐丹戈%方叶珍%龚璞%朱敏%包芳珍
張嚴峻%徐丹戈%方葉珍%龔璞%硃敏%包芳珍
장엄준%서단과%방협진%공박%주민%포방진
葡萄球菌,金黄色%肠毒素%电泳,凝胶,脉冲场
葡萄毬菌,金黃色%腸毒素%電泳,凝膠,脈遲場
포도구균,금황색%장독소%전영,응효,맥충장
Staphylococccus aureus%Enterotoxins%Electrophoresis,gel,pulsed-field
目的 通过冰淇淋及其各种原料和生产线样本分离、培养金黄色葡萄球菌,分析分离株的分子分型和表型特征,对金黄色葡萄球菌污染冰淇淋进行调查.方法 采集冰淇淋及其各种原料和生产线投料口、切片机出口、成品托盘等样本,用国标方法分离、培养、鉴定金黄色葡萄球菌,VITEK32生化验证,Mini-Vidas检测分离株肠毒素(多克隆肠毒素SEA-SEE),PCR检测分离株肠毒素基因(肠毒素SEA-SE:J基因),VITEK32测定分离菌株抗生素的耐药性,脉冲凝胶电泳(PFGE)分析菌株间的基因指纹图谱特征.结果 分别从冰淇淋和切片机出口分离到2株金黄色葡萄球菌.它们都含有肠毒素(多克隆肠毒素SEA-SEE),都有SEC、SED、SEG、SEH、SEI和SEJ肠毒素基因,耐药谱相同,脉冲凝胶电泳(PFGE)的指纹图谱相同.结论 污染冰淇淋和切片机出口的金黄色葡萄球菌是同一克隆株,切片机出口的污染导致冰淇淋污染.
目的 通過冰淇淋及其各種原料和生產線樣本分離、培養金黃色葡萄毬菌,分析分離株的分子分型和錶型特徵,對金黃色葡萄毬菌汙染冰淇淋進行調查.方法 採集冰淇淋及其各種原料和生產線投料口、切片機齣口、成品託盤等樣本,用國標方法分離、培養、鑒定金黃色葡萄毬菌,VITEK32生化驗證,Mini-Vidas檢測分離株腸毒素(多剋隆腸毒素SEA-SEE),PCR檢測分離株腸毒素基因(腸毒素SEA-SE:J基因),VITEK32測定分離菌株抗生素的耐藥性,脈遲凝膠電泳(PFGE)分析菌株間的基因指紋圖譜特徵.結果 分彆從冰淇淋和切片機齣口分離到2株金黃色葡萄毬菌.它們都含有腸毒素(多剋隆腸毒素SEA-SEE),都有SEC、SED、SEG、SEH、SEI和SEJ腸毒素基因,耐藥譜相同,脈遲凝膠電泳(PFGE)的指紋圖譜相同.結論 汙染冰淇淋和切片機齣口的金黃色葡萄毬菌是同一剋隆株,切片機齣口的汙染導緻冰淇淋汙染.
목적 통과빙기림급기각충원료화생산선양본분리、배양금황색포도구균,분석분리주적분자분형화표형특정,대금황색포도구균오염빙기림진행조사.방법 채집빙기림급기각충원료화생산선투료구、절편궤출구、성품탁반등양본,용국표방법분리、배양、감정금황색포도구균,VITEK32생화험증,Mini-Vidas검측분리주장독소(다극륭장독소SEA-SEE),PCR검측분리주장독소기인(장독소SEA-SE:J기인),VITEK32측정분리균주항생소적내약성,맥충응효전영(PFGE)분석균주간적기인지문도보특정.결과 분별종빙기림화절편궤출구분리도2주금황색포도구균.타문도함유장독소(다극륭장독소SEA-SEE),도유SEC、SED、SEG、SEH、SEI화SEJ장독소기인,내약보상동,맥충응효전영(PFGE)적지문도보상동.결론 오염빙기림화절편궤출구적금황색포도구균시동일극륭주,절편궤출구적오염도치빙기림오염.
0bjective To investigate the contamination of Staphylococccus aureus isolates in icecream by phenotypic typing and molecular typing. Methods The Staphylococccus aureus isolates were separated from ice cream, filler, cutter, salvers and material. The separated isolates were characterized by drug-resistance, staphylococcal enterotoxin (SEA-E), SE (A-E, G-J) genes and pulsed. field gelelectrophoresis (PFGE) types. Result Two Staphylococccus aureus isolates were separated. one from icecream, another from cutter. Their characteristics of drug-resistance, staphylococcal enterotoxin (SEA-E), SE(A-E, G-J) genes and PFGE type were the same. Conclusion The two Staphylococccus aureus isolates were the same clone. The contaminated Staphylococccus aureus isolates could be traced to the contaminated cutters.
