中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2010年
8期
590-594
,共5页
郑建云%任天顺%刘冰%王美妮%廖和和
鄭建雲%任天順%劉冰%王美妮%廖和和
정건운%임천순%류빙%왕미니%료화화
散发性大肠癌%hMLH1基因%hMSH2基因%免疫组织化学
散髮性大腸癌%hMLH1基因%hMSH2基因%免疫組織化學
산발성대장암%hMLH1기인%hMSH2기인%면역조직화학
Sporadic colorectal carcinoma%hMLH1 gene%hMSH2 gene%Immunohistochemistry
目的 探讨错配修复基因hMLH1和hMSH2在散发性大肠癌(SCC)组织中的表达及其临床意义.方法 采用免疫组化Max Vision二步法对63例SCC标本中的癌组织、距癌灶3 cm以外的癌旁组织、距癌灶10 cm以外的正常组织中hMLH1和hMSH2蛋白的表达进行检测.结果 hMLH1蛋白在63例正常大肠组织、癌旁组织和SCC组织中的阳性表达率分别为95.2%、85.7%和81.0%,hMLH1蛋白在SCC组织中的阳性表达率明显低于正常大肠组织(P＜0.05).hMSH2蛋白在63例正常大肠组织、癌旁组织和SCC组织中的阳性表达率分别为76.2%、66.7%和52.4%,hMSH2蛋白在SCC组织中的阳性表达率明显低于正常大肠组织(P＜0.01).hMLH1蛋白在＜60岁的SCC患者组织中的阳性表达率(100%)明显高于≥60岁的SCC患者组织(75.0%,P＜0.05),在有淋巴结转移的SCC组织中的阳性表达率(50.0%)明显低于无淋巴结转移的SCC组织(93.3%,P＜0.05).hMSH2蛋白在＜60岁的SCC患者组织中的阳性表达率(80.0%)明显高于≥60岁的SCC患者组织(43.8%,P＜0.05),在癌组织浸润至肠壁浆膜层SCC组织中的阳性表达率(61.5%)明显高于浸润至黏膜下层和肌层的SCC组织(37.5%,P＜0.05).SCC组织中hMLH1和hMSH2蛋白的表达呈正相关关系(r=0.254,P＜0.01).结论 hMLH1和hMSH2蛋白在SCC组织中的表达均有一定的缺失,并且与患者的年龄、淋巴结转移和癌组织浸润的范围有关.hMLH1和hMSH2基因可以作为临床预测和判断SCC发生和发展有用的实验室指标.
目的 探討錯配脩複基因hMLH1和hMSH2在散髮性大腸癌(SCC)組織中的錶達及其臨床意義.方法 採用免疫組化Max Vision二步法對63例SCC標本中的癌組織、距癌竈3 cm以外的癌徬組織、距癌竈10 cm以外的正常組織中hMLH1和hMSH2蛋白的錶達進行檢測.結果 hMLH1蛋白在63例正常大腸組織、癌徬組織和SCC組織中的暘性錶達率分彆為95.2%、85.7%和81.0%,hMLH1蛋白在SCC組織中的暘性錶達率明顯低于正常大腸組織(P＜0.05).hMSH2蛋白在63例正常大腸組織、癌徬組織和SCC組織中的暘性錶達率分彆為76.2%、66.7%和52.4%,hMSH2蛋白在SCC組織中的暘性錶達率明顯低于正常大腸組織(P＜0.01).hMLH1蛋白在＜60歲的SCC患者組織中的暘性錶達率(100%)明顯高于≥60歲的SCC患者組織(75.0%,P＜0.05),在有淋巴結轉移的SCC組織中的暘性錶達率(50.0%)明顯低于無淋巴結轉移的SCC組織(93.3%,P＜0.05).hMSH2蛋白在＜60歲的SCC患者組織中的暘性錶達率(80.0%)明顯高于≥60歲的SCC患者組織(43.8%,P＜0.05),在癌組織浸潤至腸壁漿膜層SCC組織中的暘性錶達率(61.5%)明顯高于浸潤至黏膜下層和肌層的SCC組織(37.5%,P＜0.05).SCC組織中hMLH1和hMSH2蛋白的錶達呈正相關關繫(r=0.254,P＜0.01).結論 hMLH1和hMSH2蛋白在SCC組織中的錶達均有一定的缺失,併且與患者的年齡、淋巴結轉移和癌組織浸潤的範圍有關.hMLH1和hMSH2基因可以作為臨床預測和判斷SCC髮生和髮展有用的實驗室指標.
목적 탐토착배수복기인hMLH1화hMSH2재산발성대장암(SCC)조직중적표체급기림상의의.방법 채용면역조화Max Vision이보법대63례SCC표본중적암조직、거암조3 cm이외적암방조직、거암조10 cm이외적정상조직중hMLH1화hMSH2단백적표체진행검측.결과 hMLH1단백재63례정상대장조직、암방조직화SCC조직중적양성표체솔분별위95.2%、85.7%화81.0%,hMLH1단백재SCC조직중적양성표체솔명현저우정상대장조직(P＜0.05).hMSH2단백재63례정상대장조직、암방조직화SCC조직중적양성표체솔분별위76.2%、66.7%화52.4%,hMSH2단백재SCC조직중적양성표체솔명현저우정상대장조직(P＜0.01).hMLH1단백재＜60세적SCC환자조직중적양성표체솔(100%)명현고우≥60세적SCC환자조직(75.0%,P＜0.05),재유림파결전이적SCC조직중적양성표체솔(50.0%)명현저우무림파결전이적SCC조직(93.3%,P＜0.05).hMSH2단백재＜60세적SCC환자조직중적양성표체솔(80.0%)명현고우≥60세적SCC환자조직(43.8%,P＜0.05),재암조직침윤지장벽장막층SCC조직중적양성표체솔(61.5%)명현고우침윤지점막하층화기층적SCC조직(37.5%,P＜0.05).SCC조직중hMLH1화hMSH2단백적표체정정상관관계(r=0.254,P＜0.01).결론 hMLH1화hMSH2단백재SCC조직중적표체균유일정적결실,병차여환자적년령、림파결전이화암조직침윤적범위유관.hMLH1화hMSH2기인가이작위림상예측화판단SCC발생화발전유용적실험실지표.
Objective To investigate the expression and clinical significance of mismatch repair genes hMLH1 and hMSH2 in sporadic colorectal carcinoma tissues. Methods The expression of hMLH1and hMSH2 proteins was detected in the 63 sporadic colorectal carcinoma samples by immunohistochemical staining, including tumor tissue,adjacent tissue at 3 cm from the carcinoma, and normal tissue at 10 cnm away from the tumor. Results The positive rate of hMLH1 protein expression in the 63 normal colorectal tissues, adjacent tissues and sporadic colorectal carcinoma tissues was 95.2%, 85.7% and 81.0%,respectively. The positive rate of hMLH1 protein expression was significantly lower in the tumor than in normal colorectal tissues ( P ＜ 0.05 ). The positive rate of hMSH2 protein in the 63 normal colorectal tissues, adjacent tissues and sporadic colorectal carcinoma tissues were 76.2%, 66.7% and 52.4%,respectively. The positive rate of hMSH2 protein expression was significantly lower in the tumor than in normal colorectal tissues (P ＜ 0.01 ). The positive rate of hMLH1 protein expression was significantly higher in the tumor tissue of patients aged younger than 60 years ( 100% ) than that in patients ≥ 60 years(75.0%,P ＜0.05) . The positive rate of hMLH1 protein expression in the tumor tissue accompanied by lymphatic metastasis was 50.0%, significantly lower than that ( 93.3% ) in tumors without lymphatic metastasis ( P ＜0.05). The positive rate of hMSH2 protein expression in the tumor tissue of patients aged younger than 60years was 80. 0%, significantly higher than that (43.8%) in the cases ≥ 60 years ( P ＜ 0.05 ). The positive rate of hMSH2 protein expression in the tumor tissues with invasion reaching to the intestinal serosa (61.5%) was significantly higher than that ( 37.5% ) in the tumors invading to submucosa or muscular layer (P ＜ 0.05 ). There was a positive correlation between the expressions of hMLH1 and hMSH2 proteins in the sporadic colorectal carcinomas. Conclusion There is a certain loss of expression of hMLH1 and hMSH2 proteins in sporadic colorectal carcinoma,and is correlated with the age of patients, lymphatic metastasis and different depth of cancer invasion. HMLH1 and hMSH2 may be used as a useful laboratory marker in clinical judgement of occurance and development of sporadic colorectal carcinoma.
