中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
41期
136-137
,共2页
张海松%孙莹%陆晓波%黄秀双
張海鬆%孫瑩%陸曉波%黃秀雙
장해송%손형%륙효파%황수쌍
铅%脂质过氧化%大鼠幼鼠%发育毒性
鉛%脂質過氧化%大鼠幼鼠%髮育毒性
연%지질과양화%대서유서%발육독성
背景:铅能促进活性氧自由基的产生,使许多组织系统处于氧化应激状态,尤其是脑组织的脂质过氧化过程.目的:通过大鼠孕期及哺乳期不同剂量染铅得到幼鼠染铅模型,观察铅对中枢神经系统的脂质过氧化作用.设计:随机对照实验.单位:辽宁省妇幼保健院和辽宁省肿瘤研究所.材料:实验于2003-06/08在中国医科大学实验室完成.选择成年Wistar大鼠150只,分笼饲养7 d后,按雌雄比2:1合笼,以次日凌晨在排泄物托盘中发现阴栓或镜检阴道分泌物发现精子定为已受孕鼠100只,并记妊娠0 d,随机将受孕鼠100只分为4组:①对照组.②饮含0.5 g/L醋酸铅水组.③饮含1 g/L醋酸铅水组.④饮含2g/L醋酸铅水组,每组25只.各实验组从受孕当天起饮用各剂量醋酸铅水;对照组饮蒸馏水5 mL,直到仔鼠生后第21天.方法:于幼鼠出生后第21天,每组取10只大鼠,在麻醉状态下断头采血,取脑组织,用于血铅、脑铅含量的测定.每组取15只大鼠,取脑组织,制成组织匀浆分别测定脂质过氧化物含量,还原型谷胱甘肽水平及超氧化物歧化酶、还原型谷胱甘肽过氧物酶、过氧化氢酶活性.主要观察指标:①出生后第21天各组幼鼠血铅、脑铅含量.②出生后第21天各组幼鼠脂质过氧化物水平,还原型谷胱甘肽含量及超氧化物歧化酶、还原型谷胱甘肽过氧物酶、过氧化氢酶活性.结果:100只幼鼠均进入结果分析.①出生后第21天各组幼鼠血铅、脑铅含量:饮含0.5,1,2g/L醋酸铅水组血铅和脑铅含量均显著高于对照组(P＜0.01),且随着染铅剂量的升高而升高.②饮含1,2 g/L醋酸铅水组脂质过氧化物水平高于对照组[(34.56±6.96),(38.76±11.11),(23.33±5.23)mmol/g,P＜0.05～0.01]、超氧化物歧化酶活性低于对照组[(423.25±157.70),(426.92±161.53),(542.78±97.69)μkat/g,P＜0.05～0.01].结论:中、高浓度重金属毒物铅可通过增强脑组织脂质过氧化过程而致发育期神经系统的损伤.
揹景:鉛能促進活性氧自由基的產生,使許多組織繫統處于氧化應激狀態,尤其是腦組織的脂質過氧化過程.目的:通過大鼠孕期及哺乳期不同劑量染鉛得到幼鼠染鉛模型,觀察鉛對中樞神經繫統的脂質過氧化作用.設計:隨機對照實驗.單位:遼寧省婦幼保健院和遼寧省腫瘤研究所.材料:實驗于2003-06/08在中國醫科大學實驗室完成.選擇成年Wistar大鼠150隻,分籠飼養7 d後,按雌雄比2:1閤籠,以次日凌晨在排洩物託盤中髮現陰栓或鏡檢陰道分泌物髮現精子定為已受孕鼠100隻,併記妊娠0 d,隨機將受孕鼠100隻分為4組:①對照組.②飲含0.5 g/L醋痠鉛水組.③飲含1 g/L醋痠鉛水組.④飲含2g/L醋痠鉛水組,每組25隻.各實驗組從受孕噹天起飲用各劑量醋痠鉛水;對照組飲蒸餾水5 mL,直到仔鼠生後第21天.方法:于幼鼠齣生後第21天,每組取10隻大鼠,在痳醉狀態下斷頭採血,取腦組織,用于血鉛、腦鉛含量的測定.每組取15隻大鼠,取腦組織,製成組織勻漿分彆測定脂質過氧化物含量,還原型穀胱甘肽水平及超氧化物歧化酶、還原型穀胱甘肽過氧物酶、過氧化氫酶活性.主要觀察指標:①齣生後第21天各組幼鼠血鉛、腦鉛含量.②齣生後第21天各組幼鼠脂質過氧化物水平,還原型穀胱甘肽含量及超氧化物歧化酶、還原型穀胱甘肽過氧物酶、過氧化氫酶活性.結果:100隻幼鼠均進入結果分析.①齣生後第21天各組幼鼠血鉛、腦鉛含量:飲含0.5,1,2g/L醋痠鉛水組血鉛和腦鉛含量均顯著高于對照組(P＜0.01),且隨著染鉛劑量的升高而升高.②飲含1,2 g/L醋痠鉛水組脂質過氧化物水平高于對照組[(34.56±6.96),(38.76±11.11),(23.33±5.23)mmol/g,P＜0.05～0.01]、超氧化物歧化酶活性低于對照組[(423.25±157.70),(426.92±161.53),(542.78±97.69)μkat/g,P＜0.05～0.01].結論:中、高濃度重金屬毒物鉛可通過增彊腦組織脂質過氧化過程而緻髮育期神經繫統的損傷.
배경:연능촉진활성양자유기적산생,사허다조직계통처우양화응격상태,우기시뇌조직적지질과양화과정.목적:통과대서잉기급포유기불동제량염연득도유서염연모형,관찰연대중추신경계통적지질과양화작용.설계:수궤대조실험.단위:요녕성부유보건원화요녕성종류연구소.재료:실험우2003-06/08재중국의과대학실험실완성.선택성년Wistar대서150지,분롱사양7 d후,안자웅비2:1합롱,이차일릉신재배설물탁반중발현음전혹경검음도분비물발현정자정위이수잉서100지,병기임신0 d,수궤장수잉서100지분위4조:①대조조.②음함0.5 g/L작산연수조.③음함1 g/L작산연수조.④음함2g/L작산연수조,매조25지.각실험조종수잉당천기음용각제량작산연수;대조조음증류수5 mL,직도자서생후제21천.방법:우유서출생후제21천,매조취10지대서,재마취상태하단두채혈,취뇌조직,용우혈연、뇌연함량적측정.매조취15지대서,취뇌조직,제성조직균장분별측정지질과양화물함량,환원형곡광감태수평급초양화물기화매、환원형곡광감태과양물매、과양화경매활성.주요관찰지표:①출생후제21천각조유서혈연、뇌연함량.②출생후제21천각조유서지질과양화물수평,환원형곡광감태함량급초양화물기화매、환원형곡광감태과양물매、과양화경매활성.결과:100지유서균진입결과분석.①출생후제21천각조유서혈연、뇌연함량:음함0.5,1,2g/L작산연수조혈연화뇌연함량균현저고우대조조(P＜0.01),차수착염연제량적승고이승고.②음함1,2 g/L작산연수조지질과양화물수평고우대조조[(34.56±6.96),(38.76±11.11),(23.33±5.23)mmol/g,P＜0.05～0.01]、초양화물기화매활성저우대조조[(423.25±157.70),(426.92±161.53),(542.78±97.69)μkat/g,P＜0.05～0.01].결론:중、고농도중금속독물연가통과증강뇌조직지질과양화과정이치발육기신경계통적손상.
BACKGROUND: lead can facilitate the active oxygen-derived free radicals formation, and make many tissue systems in oxidative stress situation especially during the process of lipid peroxidation in cerebral tissue.OBJECTIVE: To establish the dying lead model by making rat offspring to expose to lead during pregnant and breast-feed period, and observe effect of dying lead on the lipid peroxidation in central nervous system of different developmental stages,DESIGN: Randomized controlled study.SETTING: Liaoning Woman and Children Health Care College and Liaoning Tumor Institute.MATERIALS: The experiment was carried out at the Laboratory of China Medical University from June to August 2003. Totally 150 adult Wistar rats was utilized for the experiments. The ratio between female and male was 2:1. Seven days after feeding respectively, female and male rats were put together with the ratio of 2:1. The day in which the sperms were found in vaginal plug or in vaginal secretion was considered gestational day (GD)0 day. And 100 gestational rats were randomly divided into 4 groups, with solution). The lead-treated animals received solution containing lead acetate from GD 0 day, while the control animals received distilled water 5 mL from GD 0 day too, until 21 days after rat offspring giving birth.METHODS: Tissue sampling at the 21st day after rat offspring giving birth, the blood collected by head cutting of 10 rats in each group was used to determine blood lead level; brain tissue of 15 rats in each group was used to determine brain lead level, and the rest was made to be equal slurry to determine the level of lipid peroxidase (LPO), glutathione (GSH),and the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX).and activity of SOD, GSH-PX and CAT of 21-day-old rat offspring among different groups.RESULTS: Data of totally 100 infant rats was entered the final analysis.birth: Lead content of blood and brain of 21-day-old rats offspring in 0.5, 1and 2 g/L lead-treated groups were higher than those in control group observably (P ＜ 0.01), and increased along with increase of the dose of expowas higher than that in control group [(34.56±6.96), (38.76±11.11),(23.33±5.23) mmol/g, P ＜ 0.05-0.01], but activity of SOD in those two groups was lower than that in control group [(423.25±157.70),(426.92±161.53),(542.78±97.69) μkat/g, P ＜ 0.05-0.01].CONCLUSION: Lead, metal toxicant, at moderate and high dosages can induce damage of nervous system during development through accelerating lipid peroxidation of cerebral tissue.
