CAJ | 학술논문

分别以5龄幼虫的精巢和卵巢构建了2个家蚕cDNA文库.利用表达序列标签方法分析参与家蚕性腺发育的基因,通过测序获得 16 737条ESTs(精巢 8 407条,卵巢 8 330条),拼接这些ESTs共得到 2 107个重叠群(contig)和 3 532个单拷贝(singlet).将这些转录物与GenBank非冗余蛋白质库比较,结果显示约34.3%的基因与数据库中蛋白质具有相似性.功能注释表明蛋白质合成相关基因、精巢特异基因等在文库中大量存在.基因本体(gene ontology)功能分类显示精巢与卵巢基因表达谱差异较大.研究结果为理解家蚕性腺发育提供了信息.
분별이5령유충적정소화란소구건료2개가잠cDNA문고.이용표체서렬표첨방법분석삼여가잠성선발육적기인,통과측서획득 16 737조ESTs(정소 8 407조,란소 8 330조),병접저사ESTs공득도 2 107개중첩군(contig)화 3 532개단고패(singlet).장저사전록물여GenBank비용여단백질고비교,결과현시약34.3%적기인여수거고중단백질구유상사성.공능주석표명단백질합성상관기인、정소특이기인등재문고중대량존재.기인본체(gene ontology)공능분류현시정소여란소기인표체보차이교대.연구결과위리해가잠성선발육제공료신식.
Two silkworm cDNA (complementary DNA) libraries were constructed from testes and ovaries of the silkworm Bombyx mori, respectively. An expressed sequence tag (EST) analysis was performed to identify major genes involved in silkworm gonad development. 16 737 ESTs were generated by sequencing cDNA clones from the two libraries (8 407 from testis library and 8 330 from ovary library), and assembled into 2 107 contigs and 3 532 singlets. As compared to the non-redundant protein database in GenBank, approximately 34.3% of the transcripts matched proteins in the database. Functional annotations showed that genes related to protein synthesis and testis-specific proteins were enriched in both libraries. Gene ontology classification indicated that there was a remarkable difference in gene expression profiles between testes and ovaries. These results provide useful fundamental information for further studies on silkworm gonad development.