上海交通大学学报(医学版)
上海交通大學學報(醫學版)
상해교통대학학보(의학판)
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2009年
12期
1459-1462
,共4页
吴丹红%钟萍%包关水%周红%沈珺%黄菲菲
吳丹紅%鐘萍%包關水%週紅%瀋珺%黃菲菲
오단홍%종평%포관수%주홍%침군%황비비
癫%托吡酯%神经细胞黏附分子%生长相关蛋白-43
癲%託吡酯%神經細胞黏附分子%生長相關蛋白-43
전%탁필지%신경세포점부분자%생장상관단백-43
epilepsy%topiramate%nerve cell adhesion molecule%growth-associated protein 43
目的 研究不同剂量托吡酯(TPM)干预下,大鼠海马组织中神经细胞黏附分子(NCAM)和生长相关蛋白- 43(GAP- 43)mRNA的表达变化.方法 将48只大鼠随机分成正常对照组、海人酸(KA)组、10 mg/kg TPM组、40 mg/kg TPM组、100 mg/kg TPM组和400 mg/kg TPM组,每组8只;建立不同剂量的TPM干预癫大鼠模型.观察大鼠行为学改变;通过Real-time PCR测定各组大鼠海马组织中NCAM和GAP- 43的mRNA表达.结果 KA组大鼠海马NCAM及GAP- 43的mRNA表达水平明显高于正常对照组(P<0.01),10 mg/kg TPM组和40 mg/kg TPM组与KA组的差异无统计学意义,100 mg/kg TPM组和400 mg/kg TPM组明显低于KA组(P<0.01),其中400 mg/kg TPM组明显低于100 mg/kg TPM组(P<0.01).结论 KA诱导癫大鼠海马NCAM和GAP- 43 mRNA表达上调,较大剂量TPM能够抑制NCAM和GAP- 43 mRNA的表达,且抑制作用与TPM剂量有关.
目的 研究不同劑量託吡酯(TPM)榦預下,大鼠海馬組織中神經細胞黏附分子(NCAM)和生長相關蛋白- 43(GAP- 43)mRNA的錶達變化.方法 將48隻大鼠隨機分成正常對照組、海人痠(KA)組、10 mg/kg TPM組、40 mg/kg TPM組、100 mg/kg TPM組和400 mg/kg TPM組,每組8隻;建立不同劑量的TPM榦預癲大鼠模型.觀察大鼠行為學改變;通過Real-time PCR測定各組大鼠海馬組織中NCAM和GAP- 43的mRNA錶達.結果 KA組大鼠海馬NCAM及GAP- 43的mRNA錶達水平明顯高于正常對照組(P<0.01),10 mg/kg TPM組和40 mg/kg TPM組與KA組的差異無統計學意義,100 mg/kg TPM組和400 mg/kg TPM組明顯低于KA組(P<0.01),其中400 mg/kg TPM組明顯低于100 mg/kg TPM組(P<0.01).結論 KA誘導癲大鼠海馬NCAM和GAP- 43 mRNA錶達上調,較大劑量TPM能夠抑製NCAM和GAP- 43 mRNA的錶達,且抑製作用與TPM劑量有關.
목적 연구불동제량탁필지(TPM)간예하,대서해마조직중신경세포점부분자(NCAM)화생장상관단백- 43(GAP- 43)mRNA적표체변화.방법 장48지대서수궤분성정상대조조、해인산(KA)조、10 mg/kg TPM조、40 mg/kg TPM조、100 mg/kg TPM조화400 mg/kg TPM조,매조8지;건립불동제량적TPM간예전대서모형.관찰대서행위학개변;통과Real-time PCR측정각조대서해마조직중NCAM화GAP- 43적mRNA표체.결과 KA조대서해마NCAM급GAP- 43적mRNA표체수평명현고우정상대조조(P<0.01),10 mg/kg TPM조화40 mg/kg TPM조여KA조적차이무통계학의의,100 mg/kg TPM조화400 mg/kg TPM조명현저우KA조(P<0.01),기중400 mg/kg TPM조명현저우100 mg/kg TPM조(P<0.01).결론 KA유도전대서해마NCAM화GAP- 43 mRNA표체상조,교대제량TPM능구억제NCAM화GAP- 43 mRNA적표체,차억제작용여TPM제량유관.
Objective To explore the effects of different doses of topiramate (TPM) on the expression of nerve cell adhesion molecule (NCAM) and growth-associated protein 43 (GAP- 43) mRNA in hippocampus of rats with epilepsy. MethodsForty-eight rats were randomly divided into normal control group, kainic acid (KA) group, 10 mg/kg TPM group, 40 mg/kg TPM group, 100 mg/kg TPM group and 400 mg/kg TPM group (n=8). The models of rats with epilepsy treated by different doses of TPM were established. The behavior of rats was observed, and the expression of NCAM and GAP- 43 mRNA in hippocampus of rats was determined by Real-time PCR. Results The expression of NCAM and GAP- 43 mRNA in KA group was significantly higher than that in normal control group (P<0.01), while there was no significant difference between 10 and 40 mg/kg TPM groups and KA group, that in 100 and 400 mg/kg TPM groups was significantly lower than that in KA group (P<0.01), and that in 400 mg/kg TPM group was significantly lower than that in 100 mg/kg TPM group (P<0.01). Conclusion KA can up-regulate the expression of NCAM and GAP- 43 mRNA in hippocampus of rats with epilepsy. Higher dose of TPM can inhibit the expression of NCAM and GAP- 43 mRNA, and the inhibitory effect is related with the dose of TPM.