CAJ | 학술논문

目的采用流式细胞术提纯脐血嗜碱性粒细胞,以期得到研究过敏性疾病发病机制的理想标本.方法 6份足月产妇新生儿脐血经沉淀红细胞及Ficoll-泛影葡胺处理后,采用流式细胞术分选CD203c-PE~+、CD45-PerCP~(int)的细胞.通过特异性识别嗜碱性粒细胞的阿利新蓝染色和细胞计数脐血嗜碱性粒细胞的纯度和回收率.结果流式细胞术分选嗜碱性粒细胞所得的纯度为(95.02±2.94)V0,回收率为(61.42±5.95)%.结论用流式细胞术分选脐血中CD203c-PE~+、CD45-PerCP~(int)的细胞可获得高纯度、高回收率的嗜碱性粒细胞.
목적 채용류식세포술제순제혈기감성립세포,이기득도연구과민성질병발병궤제적이상표본.방법 6빈족월산부신생인제혈경침정홍세포급Ficoll-범영포알처리후,채용류식세포술분선CD203c-PE~+、CD45-PerCP~(int)적세포.통과특이성식별기감성립세포적아리신람염색화세포계수제혈기감성립세포적순도화회수솔.결과 류식세포술분선기감성립세포소득적순도위(95.02±2.94)V0,회수솔위(61.42±5.95)%.결론 용류식세포술분선제혈중CD203c-PE~+、CD45-PerCP~(int)적세포가획득고순도、고회수솔적기감성립세포.
Objective To use flow cytometry for purification of human basophils from umbilical cord blood. Methods Umbilical cord blood samples were collected from 6 healthy, full-term deliveries. After separating the red blood cells by precipitation, the mononuclear cells were isolated by FicolI-Hypaque. Then flow cytometry (FCM) was used to sort CD203c-PE~+and CD45-PerCP~(int) cells. The purity and recovery of basophils were examined by Alcian blue staining and cell counting. Results The purity and recovery of basophils by FCM were (95.02±2.94)% and (61.42±5.95)%, respectively. Conclusion Cell sorting for CD203c-PE~+,CD45- PerCP~(int) cells by FCM is a better method to obtain pure umbilicalord blood-derived basophils, with high purity and recovery.