国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
4期
305-308
,共4页
酒肝宁煎剂%酒精性肝炎%氧应激%脂质过氧化
酒肝寧煎劑%酒精性肝炎%氧應激%脂質過氧化
주간저전제%주정성간염%양응격%지질과양화
Jiuganning decoetion%Alcoholic hepatitis%Oxidative stress%Lipid peroxidation
目的 观察中药酒肝宁煎剂对酒精性肝损伤大鼠CYP2E1及细胞因子白介素-6(IL-6),肿瘤坏死因子-α(TNF-α)含量的影响.方法 清洁级Wistar雄性大鼠72只,根据大鼠体重,采用分层抽样法将大鼠分为6组,空白对照组、酒肝宁毒性试验组、酒精性肝病模型组、益肝灵治疗组、酒肝宁低剂量组、酒肝宁高剂量组.空白对照组灌胃生理盐水;酒肝宁毒性试验组灌胃23.5 g/kg酒肝宁煎剂以观察本药物有无肝脏及其他毒性;酒肝宁低剂量和高剂量组分别以生药量17.5 g/kg和23.5 g/kg灌胃:益肝灵用量为0.206 g/kg.均药物治疗4周,检测治疗前后TNF-α、IL-6、丙二醛(MDA)含量、细胞色素P4502E1(CYP2E1)和超氧化物歧化酶(SOD)活性.结果 各治疗组血清IL-6(F=46.281)、TNF-α含量(F=9.101)、肝组织CYP2E1活性(F=62.537)与酒精性肝病模型组比较,差异均有统计学意义(P均＜0.01);治疗组大鼠血清中SOD活性明显增强(F=19.210),MDA含量降低(F=34.952),与酒精性肝病模型组比较,差异均有统计学意义(P均＜0.01).结论 酒肝宁煎剂可有效降低CYP2E1及细胞因子IL-6,TNF-a含量,其抗肝损伤作用可能与抗脂质过氧化反应、调节细胞因子活性、减轻肝细胞炎症、减轻纤维化程度有关.
目的 觀察中藥酒肝寧煎劑對酒精性肝損傷大鼠CYP2E1及細胞因子白介素-6(IL-6),腫瘤壞死因子-α(TNF-α)含量的影響.方法 清潔級Wistar雄性大鼠72隻,根據大鼠體重,採用分層抽樣法將大鼠分為6組,空白對照組、酒肝寧毒性試驗組、酒精性肝病模型組、益肝靈治療組、酒肝寧低劑量組、酒肝寧高劑量組.空白對照組灌胃生理鹽水;酒肝寧毒性試驗組灌胃23.5 g/kg酒肝寧煎劑以觀察本藥物有無肝髒及其他毒性;酒肝寧低劑量和高劑量組分彆以生藥量17.5 g/kg和23.5 g/kg灌胃:益肝靈用量為0.206 g/kg.均藥物治療4週,檢測治療前後TNF-α、IL-6、丙二醛(MDA)含量、細胞色素P4502E1(CYP2E1)和超氧化物歧化酶(SOD)活性.結果 各治療組血清IL-6(F=46.281)、TNF-α含量(F=9.101)、肝組織CYP2E1活性(F=62.537)與酒精性肝病模型組比較,差異均有統計學意義(P均＜0.01);治療組大鼠血清中SOD活性明顯增彊(F=19.210),MDA含量降低(F=34.952),與酒精性肝病模型組比較,差異均有統計學意義(P均＜0.01).結論 酒肝寧煎劑可有效降低CYP2E1及細胞因子IL-6,TNF-a含量,其抗肝損傷作用可能與抗脂質過氧化反應、調節細胞因子活性、減輕肝細胞炎癥、減輕纖維化程度有關.
목적 관찰중약주간저전제대주정성간손상대서CYP2E1급세포인자백개소-6(IL-6),종류배사인자-α(TNF-α)함량적영향.방법 청길급Wistar웅성대서72지,근거대서체중,채용분층추양법장대서분위6조,공백대조조、주간저독성시험조、주정성간병모형조、익간령치료조、주간저저제량조、주간저고제량조.공백대조조관위생리염수;주간저독성시험조관위23.5 g/kg주간저전제이관찰본약물유무간장급기타독성;주간저저제량화고제량조분별이생약량17.5 g/kg화23.5 g/kg관위:익간령용량위0.206 g/kg.균약물치료4주,검측치료전후TNF-α、IL-6、병이철(MDA)함량、세포색소P4502E1(CYP2E1)화초양화물기화매(SOD)활성.결과 각치료조혈청IL-6(F=46.281)、TNF-α함량(F=9.101)、간조직CYP2E1활성(F=62.537)여주정성간병모형조비교,차이균유통계학의의(P균＜0.01);치료조대서혈청중SOD활성명현증강(F=19.210),MDA함량강저(F=34.952),여주정성간병모형조비교,차이균유통계학의의(P균＜0.01).결론 주간저전제가유효강저CYP2E1급세포인자IL-6,TNF-a함량,기항간손상작용가능여항지질과양화반응、조절세포인자활성、감경간세포염증、감경섬유화정도유관.
Objective To explore the influences of Jinganning decoction on the content of CYP2E1 and cytokine IL-6,TNF in rats models of alcohdic hepatic injury.Methos 72 male Wistar clean level rats were randomly divided into 6 groups:blank control group,Jiuganning drug toxicity test group,alcoholic hepatic disease model group,Yiganling treatment group,Jiuganning low dose group,and Jiuganning high dose group.Blank control group was treated with physiological saline;Jiuganning drug toxicity test group was treated with Jiuganning decoction to observe toxicity and side effects.Jiuganning low and high dose treatment group were treated with 17.5 g/kg and 23.5 g/kg crude drug.Yiganning treatment group were treated with 0.206 g/kg crude drug.After 4 weeks'treatment,the content of TNF,IL-6,MDA as well as the activity of CYP2E1 and SOD were measured.Results Compared with the alcoholic hepatic disease model group,the content of IL-6(F=46.281,9.101),TNF and the activity (F=62.537)of CYP2E1 of all treatment group were significantly improved(P＜0.01).The content of SOD(F=19.210)in the rats serum of all treatment group was significantly increased while the MDA(F=34.952)was decreased.All the indexes in the treatment group had significant differences with the alcoholic hepatic disease and model group(P＜0.01).Conclusions The mechanism of Jiuganning decoction can effectively reduce the content of CYP2E1 and IL-6,TNF-a,with the changes of cell physiopathology and the influences of relevant enzymes,reveals the functional routines of the decoction treating hepatic injury:anti-lipid peroxidative,adjusting cytokine's activity,protecting hepatocyte,palliating the hepatocyte inflammation,and easing fibration.
