CAJ | 학술논문

目的研究载药型晶状体囊袋张力环对体外培养猪眼晶状体囊袋模型中晶状体上皮细胞增殖的影响,探讨载药型晶状体囊袋张力环防治后发性白内障的可能性.方法连续环形撕囊晶状体超声乳化吸除术制备猪眼晶状体囊袋模型,并随机分为CTR组:植入未经处理的晶状体囊袋张力环(n=13)；CTR-PLGA-MG132组:植入表面包裹PLGA-MG132的晶状体囊袋张力环(n=13).晶状体囊袋模型体外培养3周,显微镜下方格计数法计算后囊膜细胞移行面积；晶状体囊袋模型行组织病理学检查；ELISA检测纤维连接蛋白表达最的差异.结果晶状体上皮细胞经2～3d生长潜伏期后,CTR组在(9.06±1.61)d完全覆盖后囊膜；随着培养时间的延长,后囊膜上细胞层次增加,囊膜皱褶增多；组织病理学检查可见薄层均质红染的后囊膜上被覆一层或多层排列紧密、核深染胞浆丰富的晶状体上皮细胞.而CTR-PLGA-MG132组,经4～5d生长潜伏期后,即可见晶状体上皮细胞点片状脱落,呈细小圆点状,仅残留少量细胞；1周后,细胞片状脱落、漂浮,后囊膜出现无细胞覆盖区；组织病理学检查可见均质红染的后囊膜上细胞排列松散,见无细胞覆盖区,前后囊膜平整光滑.ELISA检测CTR-PLGA-MG 132组囊袋模型纤维连接蛋白表达量[(25.14±3.73) μg/ml]显著小于CTR组[(106.86±22.34)μg/ml],差异有统计学意义(t=81.72,P＜0.01).结论相比CTR组晶状体囊袋模型,CTR-PLGA-MG132组晶状体囊袋模型残留细胞在后囊膜上移行面积、纤维连接蛋白表达均明显减少.CTR-PLGA-MG132,可有效抑制猪眼晶状体囊袋模型中晶状体上皮细胞的增殖,减少上皮-间质转化.载药型晶状体囊袋张力环是白内障术后防治后发性白内障的一种创新性尝试.
목적 연구재약형정상체낭대장력배대체외배양저안정상체낭대모형중정상체상피세포증식적영향,탐토재약형정상체낭대장력배방치후발성백내장적가능성.방법 련속배형시낭정상체초성유화흡제술제비저안정상체낭대모형,병수궤분위CTR조:식입미경처리적정상체낭대장력배(n=13)；CTR-PLGA-MG132조:식입표면포과PLGA-MG132적정상체낭대장력배(n=13).정상체낭대모형체외배양3주,현미경하방격계수법계산후낭막세포이행면적；정상체낭대모형행조직병이학검사；ELISA검측섬유련접단백표체최적차이.결과 정상체상피세포경2～3d생장잠복기후,CTR조재(9.06±1.61)d완전복개후낭막；수착배양시간적연장,후낭막상세포층차증가,낭막추습증다；조직병이학검사가견박층균질홍염적후낭막상피복일층혹다층배렬긴밀、핵심염포장봉부적정상체상피세포.이CTR-PLGA-MG132조,경4～5d생장잠복기후,즉가견정상체상피세포점편상탈락,정세소원점상,부잔류소량세포；1주후,세포편상탈락、표부,후낭막출현무세포복개구；조직병이학검사가견균질홍염적후낭막상세포배렬송산,견무세포복개구,전후낭막평정광활.ELISA검측CTR-PLGA-MG 132조낭대모형섬유련접단백표체량[(25.14±3.73) μg/ml]현저소우CTR조[(106.86±22.34)μg/ml],차이유통계학의의(t=81.72,P＜0.01).결론 상비CTR조정상체낭대모형,CTR-PLGA-MG132조정상체낭대모형잔류세포재후낭막상이행면적、섬유련접단백표체균명현감소.CTR-PLGA-MG132,가유효억제저안정상체낭대모형중정상체상피세포적증식,감소상피-간질전화.재약형정상체낭대장력배시백내장술후방치후발성백내장적일충창신성상시.
Objective Our study was performed to design a drug-sustained capsular tension ring (CTR) to evaluate its potentiality on prevention of PCO in the swine capsular bag model in vitro.Methods Following the continuous capsule curvilinear capsulorhexis ( CCCC),Phacomulsification with capsular tension ring implantation was pedormed.CTR-supported swine capsular bag models were prepared and divided into two groups,group CTR ( n =13 ) implanted with the original CTR without any modification and group CTR-PLGA-MG132 ( n =13) implanted with the CTR covered with PLGA and MG132.The CTRsupported capsular bags were cultured in vitro for up to 3 weeks.The area of lens epithelial cells (LEC) coverage over the posterior capsule surface was quantified every day under microscope.The capsules were treated for histological examination.The change of fibronectin was assessed by ELISA assay kit.Results After 2 ～ 3 days,outgrowth of LEC across the posterior capsule was observed,and the posterior capsule was totally covered by a confluent monolayer of cell after (9.06 ± 1.61 ) days in group CTR.Capsular wrinkles became increasingly apparent as time progressed.An increase in capsular thickness was also observed.In contrast,there was less LEC deposition in group CTR-PLGA-MG132.Histological examination showed LEC layers were closely arranged on the posterior capsular surface in group CTR.In group CTR-PLGA-MG132,there was comparatively looser cell arrangement.Compared with group CTR,the mean fibronectin level of posterior capsule by week 3 in group CTR-PLGA-MG132 was 25.14 μg/ml and 106.09 μg/ml respectively.Statistical analysis showed a significant difference ( P ＜ 0.01 ).Conclusions LEC migration,proliferation,and synthesis of EMT markers were inhibited in Group CTR-PLGA-MG132,compared with Group CTR.Drug-sustained capsular tension rings can effectively inhibit the migration,proliferation of LEC and the change of EMT ( epithelial-to-mesenchymal transition) in swine capsular bag models.Drug-sustained capsular tension rings might be a potential therapy to prevent the posterior capsular opacification in the future.