国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
9期
811-813
,共3页
阮月芹%韩兆东%高乐俊%孔祥华
阮月芹%韓兆東%高樂俊%孔祥華
원월근%한조동%고악준%공상화
小叶女贞%小鼠%免疫功能损伤%B淋巴细胞%NK细胞
小葉女貞%小鼠%免疫功能損傷%B淋巴細胞%NK細胞
소협녀정%소서%면역공능손상%B림파세포%NK세포
Ligustrum quihoui carr (LQC)%Mouse%Impaired immune function%CD3-CD19+ cell%CD3-CD16+CD56+ ce11
目的 探讨小叶女贞对免疫功能损伤小鼠B淋巴细胞、NK细胞免疫功能的影响。方法 以环磷酰胺致免疫功能损伤小鼠为研究对象,将BALB/C实验小鼠60只按随机数字表法随机分为5组,每组12只,分别为生理盐水(NS)对照组、环磷酰胺(Cy)对照组、Cy+小叶女贞水煎液小剂量组(小叶女贞小剂量组)、Cy+小叶女贞水煎液大剂量组(小叶女贞大剂量组)、Cy+女贞子水煎液组(女贞子组)。NS对照组给予生理盐水0.2 ml/d皮下注射10d,其余各组给予环磷酰胺0.8 ml/d皮下注射(20 mg/kg·d-1)10 d,建立免疫功能损伤小鼠模型。自第11天起各组分别给予生理盐水、环磷酰胺、小叶女贞水煎液小剂量、小叶女贞水煎液大剂量、女贞子水煎液共7天处死。用流式细胞仪检测实验鼠外周血B淋巴细胞(CD3-CD19+)和NK细胞(CD3-CD16+CD56+)的百分率。结果 CD3-CD 19+、CD3-CD16+CD56+细胞小叶女贞小剂量组[分别为(11.54±0.98)、(12.46±0.08)],明显低于小叶女贞大剂量组[分别为(20.44±1.78)、(19.12±1.70)]、女贞子组[分别为(19.90±1.42)、(20.17±1.66)]],明显高于环磷酰胺组[分别为(4.53±1.70)、(5.03±1.22)],差异均有统计学意义(P均<0.01);与NS对照组[分别为(11.84±0.99)、(12.90±0.28)]比较无明显改变(P>0.05)。结论 小叶女贞能提高小鼠B淋巴细胞和NK细胞细胞的数量,使小鼠受损伤的免疫功能得到改善,对免疫功能的调节作用与剂量成正相关。
目的 探討小葉女貞對免疫功能損傷小鼠B淋巴細胞、NK細胞免疫功能的影響。方法 以環燐酰胺緻免疫功能損傷小鼠為研究對象,將BALB/C實驗小鼠60隻按隨機數字錶法隨機分為5組,每組12隻,分彆為生理鹽水(NS)對照組、環燐酰胺(Cy)對照組、Cy+小葉女貞水煎液小劑量組(小葉女貞小劑量組)、Cy+小葉女貞水煎液大劑量組(小葉女貞大劑量組)、Cy+女貞子水煎液組(女貞子組)。NS對照組給予生理鹽水0.2 ml/d皮下註射10d,其餘各組給予環燐酰胺0.8 ml/d皮下註射(20 mg/kg·d-1)10 d,建立免疫功能損傷小鼠模型。自第11天起各組分彆給予生理鹽水、環燐酰胺、小葉女貞水煎液小劑量、小葉女貞水煎液大劑量、女貞子水煎液共7天處死。用流式細胞儀檢測實驗鼠外週血B淋巴細胞(CD3-CD19+)和NK細胞(CD3-CD16+CD56+)的百分率。結果 CD3-CD 19+、CD3-CD16+CD56+細胞小葉女貞小劑量組[分彆為(11.54±0.98)、(12.46±0.08)],明顯低于小葉女貞大劑量組[分彆為(20.44±1.78)、(19.12±1.70)]、女貞子組[分彆為(19.90±1.42)、(20.17±1.66)]],明顯高于環燐酰胺組[分彆為(4.53±1.70)、(5.03±1.22)],差異均有統計學意義(P均<0.01);與NS對照組[分彆為(11.84±0.99)、(12.90±0.28)]比較無明顯改變(P>0.05)。結論 小葉女貞能提高小鼠B淋巴細胞和NK細胞細胞的數量,使小鼠受損傷的免疫功能得到改善,對免疫功能的調節作用與劑量成正相關。
목적 탐토소협녀정대면역공능손상소서B림파세포、NK세포면역공능적영향。방법 이배린선알치면역공능손상소서위연구대상,장BALB/C실험소서60지안수궤수자표법수궤분위5조,매조12지,분별위생리염수(NS)대조조、배린선알(Cy)대조조、Cy+소협녀정수전액소제량조(소협녀정소제량조)、Cy+소협녀정수전액대제량조(소협녀정대제량조)、Cy+녀정자수전액조(녀정자조)。NS대조조급여생리염수0.2 ml/d피하주사10d,기여각조급여배린선알0.8 ml/d피하주사(20 mg/kg·d-1)10 d,건립면역공능손상소서모형。자제11천기각조분별급여생리염수、배린선알、소협녀정수전액소제량、소협녀정수전액대제량、녀정자수전액공7천처사。용류식세포의검측실험서외주혈B림파세포(CD3-CD19+)화NK세포(CD3-CD16+CD56+)적백분솔。결과 CD3-CD 19+、CD3-CD16+CD56+세포소협녀정소제량조[분별위(11.54±0.98)、(12.46±0.08)],명현저우소협녀정대제량조[분별위(20.44±1.78)、(19.12±1.70)]、녀정자조[분별위(19.90±1.42)、(20.17±1.66)]],명현고우배린선알조[분별위(4.53±1.70)、(5.03±1.22)],차이균유통계학의의(P균<0.01);여NS대조조[분별위(11.84±0.99)、(12.90±0.28)]비교무명현개변(P>0.05)。결론 소협녀정능제고소서B림파세포화NK세포세포적수량,사소서수손상적면역공능득도개선,대면역공능적조절작용여제량성정상관。
Objective To explore the effect and mechanism of LQC on the immune function of B lymphocytes and NK lymphocytes in mouse with impaired immune function. Methods Mice with impaired immune function led by Cyclophosphamide (Cy)were used as experimental animmals, and divided into five groups randomly, twelve mice in every group, which is NS control group, Cy control group, Cy+ LQC minor and major dose group, and Cy + LQC decoction group. NS control group was given NS 0.2 ml/d subcutaneously 10 d, and the rest groups were given Cy 0.8 ml/d subcutaneously (20 mg/kg · d-1) 10d, to establish a mouse model of immune dysfunction. Since the 11th day each group was given NS, Cy, LQC minor dose, major dose and fructus ligustri lucidi decoctoin. Mice of each group were killed after 7 days. The percentage of B lymphocytes (CD3 - CD19 + ) and NK lymphocytes (CD3 - CD 16 + CD56 + ) in the peripheral blood of the experimental mice were detected by flow cytometer. Results In comparison with LQC major dose group [ (20.44± 1.78)and(19.12± 1.70) ], fructus ligustri lucidi group[ (19.90± 1.42) and (20.17± 1.66) ], CD3-CD19+and CD3-CD16+CD56+ cells of LQC minor dose group[ (11.54±0.98) and (12.46±0.08)]were decreased significantly (P<0.01), which were increased significantly compared with Cy group[ (4.53± 1.70) and (5.03 ±1.22) ] (P< 0.01), but they had no significant difference with NS [ ( 11.84 ± 0.99) and ( 12.90± 0.28) ] (P > 0.05).In comparison with Cy group and NS group, CD3-CD19+ and CD3-CD16+CD56+ cells of LQC major dose group were increased significantly (P<0.01), which had no significant difference with fructus lignstri lucidi group (P>0.05). Conclusion The immune functions of the mice with impaired immune function were improved by LQC, it also could increase the quantity ofCD3-CD19+ cell and CD3-CD16+CD56+ cell. The dose of LQC was positively correlated with the modulation effect of LQC on the immune function.