CAJ | 학술논문

目的观察支气管哮喘(简称哮喘)大鼠胱硫醚-β-合成酶(cystathionine-β-synthase,CBS)mRNA及硫化氢(hydrogen sulfide,H2S)的表达变化,探讨CBS与H2S的相关性.方法采用哮喘大鼠模型,将30只SD大鼠随机分成哮喘组、对照组、地塞米松组,分光光度法测定血浆H2S含量,RT-PCR法检测肺组织CBS mRNA的表达水平.结果血浆中H2S含量哮喘组显著低干对照组,地寒米松组与哮喘组、对照组相比差异无统计学意义.肺组织CBS mRNA的表达水平哮喘组、地塞米松组显著低于对照组,地塞米松组显著高于哮喘组.肺组织CBS mRNA和血浆中H2S的表达水平呈显著正相关.结论哮喘大鼠血浆中H2S的含量和肺组织CBS mRNA的表达下降,两者呈正相关,提示可能参与了哮喘的炎症过程.地塞米松改善哮喘炎症可能部分通过H2S/CBS体系而起作用.
목적 관찰지기관효천(간칭효천)대서광류미-β-합성매(cystathionine-β-synthase,CBS)mRNA급류화경(hydrogen sulfide,H2S)적표체변화,탐토CBS여H2S적상관성.방법 채용효천대서모형,장30지SD대서수궤분성효천조、대조조、지새미송조,분광광도법측정혈장H2S함량,RT-PCR법검측폐조직CBS mRNA적표체수평.결과 혈장중H2S함량효천조현저저간대조조,지한미송조여효천조、대조조상비차이무통계학의의.폐조직CBS mRNA적표체수평효천조、지새미송조현저저우대조조,지새미송조현저고우효천조.폐조직CBS mRNA화혈장중H2S적표체수평정현저정상관.결론 효천대서혈장중H2S적함량화폐조직CBS mRNA적표체하강,량자정정상관,제시가능삼여료효천적염증과정.지새미송개선효천염증가능부분통과H2S/CBS체계이기작용.
Objective To study the expressions of endogenous hydrogen sulfide (H2S) and cystathionine-β- synthase(CBS) mRNA on acute bronchial asthma(asthma) ,and to investigate the correlation of CBS and H2S. Methods Thirty male SD rats were randomly divided into asthma group,control group,and treated group with dexamethasone (DXM). The content of H2S was detected in the plasma by spectrophotometry. The expression of CBS mRNA in the lung tissues was detected by RT-PCR. Results Compared with that of control group, the content of H2S of asthma group in the plasma significantly decreased;and there was no significance between treated group with DXM and control group, treated group with DXM and asthma group. Compared with that of control group, the expression of CBS mRNA of asthma group and treated group with DXM significantly decreased. Compared with that of asthma group, the expression of CBS mRNA of treated group with DXM significantly increased. There was significantly positive correlation between the expression of CBS mRNA in the lung tissues and the content of H2S in the plasma. Conclusions The expression of CBS mRNA in the lung tissues and the content of H2S in the plasma of asthmatic rats show decrease, there is correlation between them. They possibly participate in the inflammation process of asthma. DXM reducing the airway inflammation of asthma is possibly partly through the system of H2S/CBS.