CAJ | 학술논문

目的应用声脉冲辐射力成像(ARFI)技术观察家兔肌肉损伤后不同观察时间点肌肉组织修复的动态变化情况,并探讨其临床应用价值.方法根据不同观察时间,将16只健康家兔随机分为4组,即损伤前和损伤后1d、7d、14d.用自制的重力锤建立兔肌肉损伤模型,应用ARFI观察腓肠肌声触诊组织定量(VTQ)测值的变化.实验结束后将兔处死,取肌肉组织行病理检查.结果 ARFI示肌肉损伤后各组VTQ值明显高于正常肌肉组织,差异均具有统计学意义(P＜0.01),损伤后1d、7d、14 d组间差异均具有统计学意义(P＜0.01).病理检查结果示损伤前肌纤维连续,未见明显肿胀、出血；损伤后1d,肌纤维断裂、明显肿胀、出血、充血；损伤后7d,肌细胞大面积坏死,大量的钙盐沉积；损伤后14d,大量的纤维细胞增生,钙盐沉积明显减少.超声弹性成像与病理结果相一致.结论 ARFI可直观、无创地评价肌肉损伤后不同时间点肌肉组织修复变化,为肌肉损伤后诊断和治疗提供客观依据.
목적 응용성맥충복사력성상(ARFI)기술관찰가토기육손상후불동관찰시간점기육조직수복적동태변화정황,병탐토기림상응용개치.방법 근거불동관찰시간,장16지건강가토수궤분위4조,즉손상전화손상후1d、7d、14d.용자제적중력추건립토기육손상모형,응용ARFI관찰비장기성촉진조직정량(VTQ)측치적변화.실험결속후장토처사,취기육조직행병리검사.결과 ARFI시기육손상후각조VTQ치명현고우정상기육조직,차이균구유통계학의의(P＜0.01),손상후1d、7d、14 d조간차이균구유통계학의의(P＜0.01).병리검사결과시손상전기섬유련속,미견명현종창、출혈；손상후1d,기섬유단렬、명현종창、출혈、충혈；손상후7d,기세포대면적배사,대량적개염침적；손상후14d,대량적섬유세포증생,개염침적명현감소.초성탄성성상여병리결과상일치.결론 ARFI가직관、무창지평개기육손상후불동시간점기육조직수복변화,위기육손상후진단화치료제공객관의거.
Objective To observe the dynamic change of muscle tissue restoration after damaging at different observation time through acoustic radiation force impulse(ARFI).Methods According to different observation time,16 healthy rabbits were randomly divided into four groups,including before injury,1 day after injury,7 day after injury,14 day after injury.A homemade gravity hammer was used to establish damage model of rabbit gastrocnemius,then applied ARFI to observe changes of virtual touch tissue quantification (VTQ) of gastrocnemius.All the rabbits were executed after measurement,then the pathological changes of muscle tissue were observed under microscope.Results The VTQ of damaged muscle group were significantly higher than that of nomal muscle group( P ＜0.01),and VTQ of 1 day,7day,14 day group after injuried had significant difference between two groups (P ＜ 0.01).Through pathological examination,normal musule fibers were continual and there were not swelling and bleeding.One days after injury,muscle fibers were fractured,swollen,bleeding and congestive.7 days after injury,large areas of muscle cells were necrotic,and amounts of calcium salt depositsed.14 days after injury,lots of fiber cells proliferated,and the deposits of calcium obviously reduced.The results of ultrasound elastography were consistent with these of pathological.Conclusions ARFI can direct,noninvasively evaluate the changes of muscle tissue restoration after different time of injury,and provide objective basis for diagnosis and treatment.