中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2011年
9期
763-765
,共3页
紫癜,血小板减少性,特发性%T淋巴细胞亚群%流式细胞术
紫癜,血小闆減少性,特髮性%T淋巴細胞亞群%流式細胞術
자전,혈소판감소성,특발성%T림파세포아군%류식세포술
Purpura,thrombocytopenic,idiopathic%T-lymphocyte subsets%Flow cytometry
目的 探讨免疫细胞亚群的变化在免疫性血小板减少性紫癜(ITP)发病机制中的作用及其临床意义.方法 应用流式细胞术检测35例ITP患者治疗前、后及20例正常对照者免疫细胞亚群各指标的变化,包括CD3+、CD4+、CD8+、CD56+、CD19+淋巴细胞及CD4+/CD8+比值.结果 ITP患者CD3+ T淋巴细胞百分比(61.58±6.45)%、CD4+ T淋巴细胞百分比(28.38±4.89)%、CD4+/CD8+比值(0.99±0.22)较对照组[(67.85±4.68)%、(38.00±3.37)%、1.54±0.13]均减低(P值均＜0.05),治疗后3项指标[(69.41±5.03)%、(38.17±3.18)%、1.60±0.15]均升高至正常水平;CD8+ T淋巴细胞百分比(29.20±4.50)%及CD19+ B淋巴细胞百分比(17.74±4.14)%较对照组[(24.82±2.93)%、(12.09±3.51)%]升高(P值均＜0.05),治疗后2项指标[(24.06±3.02)%、(10.90±3.55)%]均降至正常水平;ITP患者CD56+细胞百分比治疗前(15.80±2.85)%、治疗后(15.16±2.77)%与对照组(16.36±2.75)%差异无统计学意义(P＞0.05).结论 免疫细胞亚群紊乱参与了ITP的发病,对其检测可作为ITP的辅助诊断,在指导治疗方面可能有一定的意义.
目的 探討免疫細胞亞群的變化在免疫性血小闆減少性紫癜(ITP)髮病機製中的作用及其臨床意義.方法 應用流式細胞術檢測35例ITP患者治療前、後及20例正常對照者免疫細胞亞群各指標的變化,包括CD3+、CD4+、CD8+、CD56+、CD19+淋巴細胞及CD4+/CD8+比值.結果 ITP患者CD3+ T淋巴細胞百分比(61.58±6.45)%、CD4+ T淋巴細胞百分比(28.38±4.89)%、CD4+/CD8+比值(0.99±0.22)較對照組[(67.85±4.68)%、(38.00±3.37)%、1.54±0.13]均減低(P值均＜0.05),治療後3項指標[(69.41±5.03)%、(38.17±3.18)%、1.60±0.15]均升高至正常水平;CD8+ T淋巴細胞百分比(29.20±4.50)%及CD19+ B淋巴細胞百分比(17.74±4.14)%較對照組[(24.82±2.93)%、(12.09±3.51)%]升高(P值均＜0.05),治療後2項指標[(24.06±3.02)%、(10.90±3.55)%]均降至正常水平;ITP患者CD56+細胞百分比治療前(15.80±2.85)%、治療後(15.16±2.77)%與對照組(16.36±2.75)%差異無統計學意義(P＞0.05).結論 免疫細胞亞群紊亂參與瞭ITP的髮病,對其檢測可作為ITP的輔助診斷,在指導治療方麵可能有一定的意義.
목적 탐토면역세포아군적변화재면역성혈소판감소성자전(ITP)발병궤제중적작용급기림상의의.방법 응용류식세포술검측35례ITP환자치료전、후급20례정상대조자면역세포아군각지표적변화,포괄CD3+、CD4+、CD8+、CD56+、CD19+림파세포급CD4+/CD8+비치.결과 ITP환자CD3+ T림파세포백분비(61.58±6.45)%、CD4+ T림파세포백분비(28.38±4.89)%、CD4+/CD8+비치(0.99±0.22)교대조조[(67.85±4.68)%、(38.00±3.37)%、1.54±0.13]균감저(P치균＜0.05),치료후3항지표[(69.41±5.03)%、(38.17±3.18)%、1.60±0.15]균승고지정상수평;CD8+ T림파세포백분비(29.20±4.50)%급CD19+ B림파세포백분비(17.74±4.14)%교대조조[(24.82±2.93)%、(12.09±3.51)%]승고(P치균＜0.05),치료후2항지표[(24.06±3.02)%、(10.90±3.55)%]균강지정상수평;ITP환자CD56+세포백분비치료전(15.80±2.85)%、치료후(15.16±2.77)%여대조조(16.36±2.75)%차이무통계학의의(P＞0.05).결론 면역세포아군문란삼여료ITP적발병,대기검측가작위ITP적보조진단,재지도치료방면가능유일정적의의.
Objective To explore the clinical significance of immunocyte subsets before and after immunosuppressive therapy in the peripheral blood of patients with immune thrombocytopenic purpura (ITP).MethodsThe percentages of immunocyte subsets in the peripheral blood of 35 patients with ITP and 20 healthy controls were detected by flow cytometry,including CD3+,CD4+,CD8+,CD56+,CD19+ lymphocytes and CD4+/CD8+.Results The percentages of CD3+ T lymphocyte (61.58 ± 6.45 ) %,CD4+ T lymphocyte (28.38 ±4.89)% and the ratio of CD4+/CD8+ 0.99 0.22 in patients with ITP were lower than those in healthy controls[( 67.85 ± 4.68 ) %,( 38.00 ± 3.37 ) %,1.54 ± 0.13,all P ＜ 0.05].After immunosuppressive therapy,the percentages of CD3+ T lymphocyte ( 69.41 ± 5.03 ) %,CD4+ T lymphocyte (38.17 ±3.18)% and the ratio of CD4+/CD8+ 1.60 ±0.15 recovered to control levels.The percentages of CD8+ T lymphocyte (29.20 ±4.50)% and CD19+B lymphocyte ( 17.74 ±4.14)% were higher than those in healthy controls[( 24.82 ± 2.93 ) % and ( 12.09 ± 3.51 ) %,all P ＜ 0.05].After the immunosuppressive therapy,the percentages of CD8+ T lymphocyte ( 24.06 ± 3.02 ) % and CD19+ B lymphocyte ( 10.90 ± 3.55 ) %recovered to control levels.There were no significant difference of the percentage of CD56+ lymphocyte among ITP patients ( 15.80 ± 2.85 )%,ITP patients after immunosuppressive therapy ( 15.16 ± 2.77 )% and healthy controls ( 16.36 ± 2.75 ) %.ConclusionThe aberrant immunocyte subsets are involved in the pathogenesis of ITP,and detection of immunocyte subsets might be helpful for the diagnosis and determination of therapeutic outcome of ITP.
