苏云金芽孢杆菌菌株YBT1535转cry1C基因菌的构建
소운금아포간균균주YBT1535전cry1C기인균적구건
Transfer of cry1C Gene into Bacillus thuringiensis by Electroporation to Construct Strain with Broader Insecticidal Activity
  • 万方数据
    生物工程学报 생물공정학보
    CHINESE JOURNAL OF BIOTECHNOLOGY
    2000年 5期 587-590 ,共4页

    鲁松清%刘子铎%喻子牛

    로송청%류자탁%유자우

    苏云金芽孢杆菌%基因cry1C%电脉冲转化 囌雲金芽孢桿菌%基因cry1C%電脈遲轉化
    소운금아포간균%기인cry1C%전맥충전화
    利用电脉冲将cry1C基因转入苏云金芽孢杆菌野生菌株YBT1535,筛选得到3个转化子.质粒电泳、PCR扩增及Southern杂交结果均证明,基因cry1C已转入菌株YBT1535.生物测定结果表明,3个转化子对甜菜夜蛾的毒力比出发苗YBT1535均有显著的提高,转化子YBT1535-1和YBT1515-3对小菜蛾和棉铃虫的生物活性与出发菌YBT1535相近,而转化子YBT1535-2则有一定幅度的提高.
    이용전맥충장cry1C기인전입소운금아포간균야생균주YBT1535,사선득도3개전화자.질립전영、PCR확증급Southern잡교결과균증명,기인cry1C이전입균주YBT1535.생물측정결과표명,3개전화자대첨채야아적독력비출발묘YBT1535균유현저적제고,전화자YBT1535-1화YBT1515-3대소채아화면령충적생물활성여출발균YBT1535상근,이전화자YBT1535-2칙유일정폭도적제고.
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