中国药科大学学报
中國藥科大學學報
중국약과대학학보
JOURNAL OF CHINA PHARMACEUTICAL UNIVERSITY
2004年
3期
271-275
,共5页
莫达非尼%过氧化氢%PC12细胞%凋亡%流式细胞术%线粒体膜电位
莫達非尼%過氧化氫%PC12細胞%凋亡%流式細胞術%線粒體膜電位
막체비니%과양화경%PC12세포%조망%류식세포술%선립체막전위
Modafinil%Hydrogen peroxide%PC12 cell%Apoptosis%Flow cytometry%Mitochondrial membrane potential
目的:研究莫达非尼(modafinil)对过氧化氢诱导的PC12细胞凋亡的抑制作用,并对其机制进行探讨.方法:以噻唑兰(MTT)法测定PC12细胞存活率;用流式细胞术检测PC12细胞凋亡的百分率;测定细胞内罗丹明123(Rhodamine123)的荧光强度,反映细胞线粒体膜电位的改变.结果:莫达非尼能抑制过氧化氢(200 μmol/L)、硝普钠(500μmol/L)和连二亚硫酸钠(2 mmol/L)对PC12细胞的损伤.过氧化氢(200 μmol/L)24h可以诱导PC12细胞凋亡(凋亡率为32.65%).莫达非尼(15,7.5 μmol/L)显著降低凋亡细胞的百分率(凋亡百分率分别为7.95%、15.46%).并且莫达非尼可以抑制过氧化氢引起的线粒体膜电位降低.结论:莫达非尼对PC12细胞有保护作用.能抑制过氧化氢诱导的PC12细胞凋亡,这一作用可能与其抑制过氧化氢引起的线粒体膜电位降低有关.
目的:研究莫達非尼(modafinil)對過氧化氫誘導的PC12細胞凋亡的抑製作用,併對其機製進行探討.方法:以噻唑蘭(MTT)法測定PC12細胞存活率;用流式細胞術檢測PC12細胞凋亡的百分率;測定細胞內囉丹明123(Rhodamine123)的熒光彊度,反映細胞線粒體膜電位的改變.結果:莫達非尼能抑製過氧化氫(200 μmol/L)、硝普鈉(500μmol/L)和連二亞硫痠鈉(2 mmol/L)對PC12細胞的損傷.過氧化氫(200 μmol/L)24h可以誘導PC12細胞凋亡(凋亡率為32.65%).莫達非尼(15,7.5 μmol/L)顯著降低凋亡細胞的百分率(凋亡百分率分彆為7.95%、15.46%).併且莫達非尼可以抑製過氧化氫引起的線粒體膜電位降低.結論:莫達非尼對PC12細胞有保護作用.能抑製過氧化氫誘導的PC12細胞凋亡,這一作用可能與其抑製過氧化氫引起的線粒體膜電位降低有關.
목적:연구막체비니(modafinil)대과양화경유도적PC12세포조망적억제작용,병대기궤제진행탐토.방법:이새서란(MTT)법측정PC12세포존활솔;용류식세포술검측PC12세포조망적백분솔;측정세포내라단명123(Rhodamine123)적형광강도,반영세포선립체막전위적개변.결과:막체비니능억제과양화경(200 μmol/L)、초보납(500μmol/L)화련이아류산납(2 mmol/L)대PC12세포적손상.과양화경(200 μmol/L)24h가이유도PC12세포조망(조망솔위32.65%).막체비니(15,7.5 μmol/L)현저강저조망세포적백분솔(조망백분솔분별위7.95%、15.46%).병차막체비니가이억제과양화경인기적선립체막전위강저.결론:막체비니대PC12세포유보호작용.능억제과양화경유도적PC12세포조망,저일작용가능여기억제과양화경인기적선립체막전위강저유관.
AIM: To study the inhibition of hydrogen peroxide-induced PC12 cell apoptosis by modafinil and its mechanism. METHOD: PC12 cell survival was measured by MTT assay. The percentage of apoptosis was monitored with flow cytometry. By measuring the intracellular rhodamine123(Rh123)fluorescence density,we evaluated the change of PC12 cell mitochondrial membrane potential (mmp) induced by hydrogen peroxide. RESULT:Modafinil attenuated the cytotoxic effect of hydrogen peroxide (200 μmol/L),sodium nitoprusside (500 μmol/L) and sodium dithionite (2mmol/L). Hydrogen peroxide (200 μmol/L,24 h) induced apoptosis in PC12 cells.PC12 cells were preconditioned with modafinil, then the percentage of apoptosis in the cells was decreased significantly to 7.95%,15.46%,and 17.52% from 32.65%. Modafinil inhibited the mmp decrease in PC12 cells induced by hydrogen peroxide. CONCLUSION:Modafinil prevented PC12 cell from apoptosis induced by hydrogen peroxide,and the mechanism might be associated with its effect of increasing the mitochondrial membrane potential of PC12 cell.
