CAJ | 학술논문

目的:建立一种持续时间较久的慢性高眼压动物模型.方法:新西兰白兔10只左眼为对照眼,右眼为模型眼.模型眼前房注入3g/L复方卡波姆溶液制作成慢性高眼压模型,对照眼只作前房穿刺.每日测量眼压;在高眼压模型前和高眼压持续1,2及4wk共4个时间点,检测ERG的b波、Ops波的振幅;高眼压持续4wk后检测视网膜节细胞(retinalganglioncells,RGCs)数目的变化.结果:①模型眼的眼压(4.47±1.41)kPa,明显高于对照眼(2.08±0.25)kPa(t=4.798,P=0.001).②高眼压持续1,2及4wk后,对照眼b波的振幅为(211.49±57.22,207.86±51.89,201.77±56.47)μV,Ops波的振幅为(77.45±24.08,73.61±8.87,79.66±21.94)μV;模型眼b波的振幅为(149.08±20.55,121.12±27.99,81.82±77.66)μV,Ops波的振幅为(50.09±9.77,39.99±8.98,33.12±7.67)μV;二者间b波、Ops波的振幅有非常显著性差异(P值均为0.000),模型眼b波和Ops波的振幅在高眼压前和高眼压持续1,2及4wk时有非常显著性差异(P值均为0.000),并且随高眼压持续时间的延长有进行性降低的趋势(P值分别为0.001,0.000).③慢性高眼压持续4wk后检测RGCs数目对照眼为(302.30±23.99)/10mm和模型眼为(89.20±10.86)/10mm,有非常显著性差异(t=23.685,P=0.000).结论:从眼压、视网膜功能和形态来衡量,兔眼前房注射3g/L复方卡波姆溶液是一种理想的慢性高眼压模型.
목적:건립일충지속시간교구적만성고안압동물모형.방법:신서란백토10지좌안위대조안,우안위모형안.모형안전방주입3g/L복방잡파모용액제작성만성고안압모형,대조안지작전방천자.매일측량안압;재고안압모형전화고안압지속1,2급4wk공4개시간점,검측ERG적b파、Ops파적진폭;고안압지속4wk후검측시망막절세포(retinalganglioncells,RGCs)수목적변화.결과:①모형안적안압(4.47±1.41)kPa,명현고우대조안(2.08±0.25)kPa(t=4.798,P=0.001).②고안압지속1,2급4wk후,대조안b파적진폭위(211.49±57.22,207.86±51.89,201.77±56.47)μV,Ops파적진폭위(77.45±24.08,73.61±8.87,79.66±21.94)μV;모형안b파적진폭위(149.08±20.55,121.12±27.99,81.82±77.66)μV,Ops파적진폭위(50.09±9.77,39.99±8.98,33.12±7.67)μV;이자간b파、Ops파적진폭유비상현저성차이(P치균위0.000),모형안b파화Ops파적진폭재고안압전화고안압지속1,2급4wk시유비상현저성차이(P치균위0.000),병차수고안압지속시간적연장유진행성강저적추세(P치분별위0.001,0.000).③만성고안압지속4wk후검측RGCs수목대조안위(302.30±23.99)/10mm화모형안위(89.20±10.86)/10mm,유비상현저성차이(t=23.685,P=0.000).결론:종안압、시망막공능화형태래형량,토안전방주사3g/L복방잡파모용액시일충이상적만성고안압모형.