背景:黄芩是一味常用中药,传统药用其根,茎叶一0是被废弃,为充分利用黄芩茎叶资源,本研究所对黄芩茎叶的药理药化进行了系统研究.
目的:探讨黄芩茎叶的主要药效部位总黄酮对大鼠实验性高脂血症的预防作用.
设计:随机对照实验.
单位:承德医学院中药研究所.
对象:实验于1999-03/2000-01在承德医学院中药研究所完成.wistar大鼠60只,雄性,初始体质量(200±10)g,由中国医学科学院实验动物繁育场提供,合格证号:01-3008.
干预:大鼠60只被随机分为6组,每组10只,正常对照组,高脂模型组,总黄酮小、中、大剂量组(剂量分别为12.5,25,50 mg/kg)和氯苯丁酯组(剂量为25 mg/kg).正常对照组饲喂基础饲料,高脂模型组饲喂高脂饲料,总黄酮大、中、小剂量组和氯苯丁酯组,在喂高脂饲料的同时给予相应剂量的药物.连续给药30 d,观察大鼠的血脂变化.
主要观察指标:应用CL-7200型全自动生化分析仪,检测大鼠血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇的含量,并计算动脉粥样硬化指数(总胆固醇-高密度脂蛋白/高密度脂蛋白).
结果:纳入60只大鼠均进入结果分析.①大鼠血清总胆固醇含量:高脂模型组明显高于正常对照组[(5.01±1.05,2.33±0.35)mmol/L,(P＜0.01)];总黄酮小、中、大剂量组分别为(4.15±1.12,3.03±0.31,2.98±0.56)mmol/L,小剂量组与模型组比较差异无显著性(t=1.74,P＞0.05),中、大剂量组与模型组比较差异显著(t=5.66～5.23,P＜0.01).②大鼠血清三酰甘油的含量:总黄酮小、中、大剂量组分别为[(1.22±0.56,1.56±0.41,1.24±0.45)mmol/L],与模型组[(2.14±0.74)mmol/L]比较差异显著(t=2.19～3.45,P＜0.05～0.01).③大鼠血清低密度脂蛋白胆固醇含量:总黄酮小、中、大剂量组分别为[(2.67±0.45,1.41±0.23,1.29±0.23)mmol/L],与模型组[(3.94±0.42)mmol/L]比较差异显著(t=5.77～12.71,P＜0.05～0.01].④大鼠血清高密度脂蛋白胆固醇含量:总黄酮小、中、大剂量组分别为[(0.72±0.23,0.91±0 32,1.05±0.23)mmol/L],小剂量组与模型组[(0.56±0.21)mmol/L]比较差异无显著性(t=1.51,P＞0.05),中、大剂量组与模型组比较差异显著(t=2.92～4.38,P＜0.05～0.01).⑤动脉粥样硬化指数:总黄酮小、中、大剂量组分别为(2.96±1.35,2.10±0.97,1.55±0.41),与模型组(4.23±0.65)比较差异显著(t=3.54～9.49,P＜0.01).
结论:黄芩茎叶总黄酮对大鼠长期喂以高脂饲料所造成的血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇的升高有明显的抑制作用,并使高密度脂蛋白胆固醇的含量有一定程度的升高,表明总黄酮对大鼠实验性高脂血症有明显的预防作用.
揹景:黃芩是一味常用中藥,傳統藥用其根,莖葉一0是被廢棄,為充分利用黃芩莖葉資源,本研究所對黃芩莖葉的藥理藥化進行瞭繫統研究.
目的:探討黃芩莖葉的主要藥效部位總黃酮對大鼠實驗性高脂血癥的預防作用.
設計:隨機對照實驗.
單位:承德醫學院中藥研究所.
對象:實驗于1999-03/2000-01在承德醫學院中藥研究所完成.wistar大鼠60隻,雄性,初始體質量(200±10)g,由中國醫學科學院實驗動物繁育場提供,閤格證號:01-3008.
榦預:大鼠60隻被隨機分為6組,每組10隻,正常對照組,高脂模型組,總黃酮小、中、大劑量組(劑量分彆為12.5,25,50 mg/kg)和氯苯丁酯組(劑量為25 mg/kg).正常對照組飼餵基礎飼料,高脂模型組飼餵高脂飼料,總黃酮大、中、小劑量組和氯苯丁酯組,在餵高脂飼料的同時給予相應劑量的藥物.連續給藥30 d,觀察大鼠的血脂變化.
主要觀察指標:應用CL-7200型全自動生化分析儀,檢測大鼠血清總膽固醇、三酰甘油、低密度脂蛋白膽固醇、高密度脂蛋白膽固醇的含量,併計算動脈粥樣硬化指數(總膽固醇-高密度脂蛋白/高密度脂蛋白).
結果:納入60隻大鼠均進入結果分析.①大鼠血清總膽固醇含量:高脂模型組明顯高于正常對照組[(5.01±1.05,2.33±0.35)mmol/L,(P＜0.01)];總黃酮小、中、大劑量組分彆為(4.15±1.12,3.03±0.31,2.98±0.56)mmol/L,小劑量組與模型組比較差異無顯著性(t=1.74,P＞0.05),中、大劑量組與模型組比較差異顯著(t=5.66～5.23,P＜0.01).②大鼠血清三酰甘油的含量:總黃酮小、中、大劑量組分彆為[(1.22±0.56,1.56±0.41,1.24±0.45)mmol/L],與模型組[(2.14±0.74)mmol/L]比較差異顯著(t=2.19～3.45,P＜0.05～0.01).③大鼠血清低密度脂蛋白膽固醇含量:總黃酮小、中、大劑量組分彆為[(2.67±0.45,1.41±0.23,1.29±0.23)mmol/L],與模型組[(3.94±0.42)mmol/L]比較差異顯著(t=5.77～12.71,P＜0.05～0.01].④大鼠血清高密度脂蛋白膽固醇含量:總黃酮小、中、大劑量組分彆為[(0.72±0.23,0.91±0 32,1.05±0.23)mmol/L],小劑量組與模型組[(0.56±0.21)mmol/L]比較差異無顯著性(t=1.51,P＞0.05),中、大劑量組與模型組比較差異顯著(t=2.92～4.38,P＜0.05～0.01).⑤動脈粥樣硬化指數:總黃酮小、中、大劑量組分彆為(2.96±1.35,2.10±0.97,1.55±0.41),與模型組(4.23±0.65)比較差異顯著(t=3.54～9.49,P＜0.01).
結論:黃芩莖葉總黃酮對大鼠長期餵以高脂飼料所造成的血清總膽固醇、三酰甘油、低密度脂蛋白膽固醇的升高有明顯的抑製作用,併使高密度脂蛋白膽固醇的含量有一定程度的升高,錶明總黃酮對大鼠實驗性高脂血癥有明顯的預防作用.
배경:황금시일미상용중약,전통약용기근,경협일0시피폐기,위충분이용황금경협자원,본연구소대황금경협적약리약화진행료계통연구.
목적:탐토황금경협적주요약효부위총황동대대서실험성고지혈증적예방작용.
설계:수궤대조실험.
단위:승덕의학원중약연구소.
대상:실험우1999-03/2000-01재승덕의학원중약연구소완성.wistar대서60지,웅성,초시체질량(200±10)g,유중국의학과학원실험동물번육장제공,합격증호:01-3008.
간예:대서60지피수궤분위6조,매조10지,정상대조조,고지모형조,총황동소、중、대제량조(제량분별위12.5,25,50 mg/kg)화록분정지조(제량위25 mg/kg).정상대조조사위기출사료,고지모형조사위고지사료,총황동대、중、소제량조화록분정지조,재위고지사료적동시급여상응제량적약물.련속급약30 d,관찰대서적혈지변화.
주요관찰지표:응용CL-7200형전자동생화분석의,검측대서혈청총담고순、삼선감유、저밀도지단백담고순、고밀도지단백담고순적함량,병계산동맥죽양경화지수(총담고순-고밀도지단백/고밀도지단백).
결과:납입60지대서균진입결과분석.①대서혈청총담고순함량:고지모형조명현고우정상대조조[(5.01±1.05,2.33±0.35)mmol/L,(P＜0.01)];총황동소、중、대제량조분별위(4.15±1.12,3.03±0.31,2.98±0.56)mmol/L,소제량조여모형조비교차이무현저성(t=1.74,P＞0.05),중、대제량조여모형조비교차이현저(t=5.66～5.23,P＜0.01).②대서혈청삼선감유적함량:총황동소、중、대제량조분별위[(1.22±0.56,1.56±0.41,1.24±0.45)mmol/L],여모형조[(2.14±0.74)mmol/L]비교차이현저(t=2.19～3.45,P＜0.05～0.01).③대서혈청저밀도지단백담고순함량:총황동소、중、대제량조분별위[(2.67±0.45,1.41±0.23,1.29±0.23)mmol/L],여모형조[(3.94±0.42)mmol/L]비교차이현저(t=5.77～12.71,P＜0.05～0.01].④대서혈청고밀도지단백담고순함량:총황동소、중、대제량조분별위[(0.72±0.23,0.91±0 32,1.05±0.23)mmol/L],소제량조여모형조[(0.56±0.21)mmol/L]비교차이무현저성(t=1.51,P＞0.05),중、대제량조여모형조비교차이현저(t=2.92～4.38,P＜0.05～0.01).⑤동맥죽양경화지수:총황동소、중、대제량조분별위(2.96±1.35,2.10±0.97,1.55±0.41),여모형조(4.23±0.65)비교차이현저(t=3.54～9.49,P＜0.01).
결론:황금경협총황동대대서장기위이고지사료소조성적혈청총담고순、삼선감유、저밀도지단백담고순적승고유명현적억제작용,병사고밀도지단백담고순적함량유일정정도적승고,표명총황동대대서실험성고지혈증유명현적예방작용.
BACKGROUND: The Scutellaria baicalensis is a traditional Chinese herb frequently used. Only its root is used, and its stem and leaf are abandoned in traditional custom. Inorder to make full use of medicinal material resource, the chemical component and pharmacological effects of the stem and leaf have been studied.
OBJECTIVE: To observe the preventive effect of total flavones from stem and leaf of scutellaria baicalensis on experimental hyperlipidemia in rats.
DESIGN: Randomized controlled trial.
SETTING: Institute of Traditional Chinese Medicine, Chengde Medical College
MATERIALS: The experiment was conducted in the Institute of Traditional Chinese Medicine, Chengde Medical College from March 1999 to January 2000. Totally 60 male Wistar rats with beginning body mass(200±10)g were provided by Experimental Animal Research Institute, Chinese Academy of Medical Science (Qualification No.01-3008).
INTERVENTIONS: Sixty rats were randomly divided into 6 groups:normal control group, high-lipid model group, the groups of 12.5, 25 and 50 mg/kg of total flavonoids (TF group) and clofibrate group (25 mg/kg),with 10 rats in each group. The rats in the normal control group were fed with basic feed. The rats in the high-lipid model group were fed with highlipid feed. Rats in the TF group and clofibrate group were fed synchronously with high-lipid feed and total flavonoids or clofibrate for consecutive 30 days. The change of blood lipid was observed.
MAIN OUTCOME MEASURES: The levels of total cholesterol (TC),triglyceride (TG), low density lipoprotein (LDL-C), high density lipoprotein (HDL-C) in the serum of all the rats were measured with CL-7200 type automatic biochemistry analytical instrument at the end of the experiment, and the atherosclerosis indexes (AI) were calculated (AI=TC-HDL-C/HDL-C).RESULTS: Totally 60 rats entered the result analysis. ① Level of TC in the serum of rats: The level in the high-lipid model group was significantly higher than that in the normal control group [(5.01 ±1.05,2.33±0.35)mmol/L, (P ＜ 0.01 )]; The level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was (4.15±1.12, 3.03±0.31,2.98±0.56)mmol/L, there was no significant difference between group of 12.5 mg/kg of TF and model group (t=1.74, P ＞ 0.05), but there was significant difference between groups of 25 mg/kg and 50 mg/kg of TF and model group (t=5.66-5.23, P ＜ 0.01). ②Level of TG in the serum of rats: The level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was (1.22±0.56)mmol/L,(1.56±0.41)mmol/L,(1.24 ±0.45)mmol/L respectively, compared with model group(2.14±0.74) mmol/L, there was significant difference (t =2.19-3.45, P ＜ 0.05-0.01). ③ LDL-C level of the serum in the rats: The level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was (2.67 ±0.45) mmol/L, (1.41 ±0.23)mmol/L, (1.29±0.23) mmol/Lrespcrtively,compared with model group[(3.94±0.42)mmol/L, there was significant difference (t=5.77-12.71, P ＜ 0.05-0.01). ④ HDL-C level of the serum in the rats: the level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was (0.72±0.23)mmol/L,( 0.91±0.32)mmoL/L,(1.05±0.23)mmoL/L respectively, there was no significant difference between group of 12.5 mg/kg of TF and model group[(0.56±0.21)mmol/L, but there was significant difference between groups of 25 mg/kg and 50 mg/kg of TF and model group (t=2.92-4.38,0.05 ,P ＜ 0.05-0.01).⑤AI: the level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was(2.96 ±1.35), (2.10±0.97), (1.55±0.41)respectively, compared with model group (4.23±0.65) , there was significant difference (t =3.54-9.49 ,P ＜ 0.01).
CONCLUSION: TF has significantly inhibitory effect on the increase of TC, TG and LDL-C in the serum of the rats induced by high-lipid feed; it can also increase the level of HDL-C, indicating TF has obviously preventive effect on experimental hyperlipidemia in rats.
