CAJ | 학술논문

回顾腔前卵泡体外分离方法的研究历史,着重论述牛、羊、猪等家畜的腔前卵泡体外分离方法及其影响因素,以期为建立一种更为有效的分离方法提供理论基础.
회고강전란포체외분리방법적연구역사,착중논술우、양、저등가축적강전란포체외분리방법급기영향인소,이기위건립일충경위유효적분리방법제공이론기출.
In the mammalian ovary, about 99% of the follicles present in the cortex are in the form of a non-cavity. It is essential for isolating preantral follicles effectively. The separation of follicles is established in rodents. However, this remains a challenge in livestock which has constrained research. The separation methods for livestock animals preantral follicles can be largely divided into three categories: enzymatic digestion method, mechanical separation, combined enzyme-mechanical separation. According to the different tools researchers use, mechanical separation is generally divided into the following categories: cutting method, comb-scrape method, micromanipulation, and grinding. This article reviews the methodological history of isolating preantral follicles in vitro, focuses on the method for isolation of preantral follicles in vitro and influencing factors, such as animal species, age, processing time, and ovarian size and development, thus laying a theoretical foundation for establishing a more effective isolation method.